We present an analysis of seven strongly gravitationally lensed quasars and the corresponding constraints on the properties of dark matter. Our results are derived by modelling the lensed image positions and flux-ratios using a combination of smooth macro-models and a population of low-mass haloes within the mass range of 10⁶–10⁹�M_⊙. Our lens models explicitly include higher order complexity in the form of stellar discs and luminous satellites, as well as low-mass haloes located along the observed lines of sight for the first time. Assuming a cold dark matter (CDM) cosmology, we infer an average total mass fraction in substructure of (68�per cent confidence limits), which is in agreement with the predictions from CDM hydrodynamical simulations to within 1σ. This result is closer to the predictions than those from previous studies that did not include line-of-sight haloes. Under the assumption of a thermal relic dark matter model, we derive a lower limit on the particle relic mass of m_th > 5.58�keV (95�per cent confidence limits), which is consistent with a value of m_th > 5.3�keV from the recent analysis of the Ly�α forest. We also identify two main sources of possible systematic errors and conclude that deeper investigations in the complex structure of lens galaxies as well as the size of the background sources should be a priority for this field.