Centrosomes are microtubule-organizing centres of animal cells. They influence the
morphology of the microtubule cytoskeleton, function as the base for the primary cilium and�…

The number, length, distribution and polarity of microtubules are largely controlled by
microtubule-organizing centres, which nucleate and anchor microtubule minus ends in a�…

Publisher Summary This chapter reviews and provides detailed protocols for the application
of immunofluorescence and other fluorescence-microscopic procedures to yeast. These�…

We have cloned and characterized y-tubulin genes from both X. laevis and S. pombe, and
partial genes from maize, diatom, and a budding yeast. The proteins encoded by these�…

The centrosome organizes the microtubule cytoskeleton and consists of a pair of centrioles
surrounded by pericentriolar material. Cells begin the cell cycle with a single centrosome�…

The centrosome nucleates microtubule polymerization, affecting mkrotubule number,
polarity, and structure. We use an in vitro system based on extmcts of Xenopus eggs to�…

Primary cilia are sensory organelles that translate extracellular chemical and mechanical
cues into cellular responses. Bone is an exquisitely mechanosensitive organ, and its�…

ADP-ribosylation factor (ARF) is a ubiquitous, highly conserved 21-kDa GTP-binding protein,
first identified in animal cells as the cofactor required for the in vitro ADP-ribosylation of the�…

Proper orientation of the mitotic spindle is critical for successful cell division in budding
yeast. To investigate the mechanism of spindle orientation, we used a green fluorescent�…

This paper examines evidence concerning the existence of organizational buffers, which
insulate an organization from environmental disturbances, and transformational shields�…