Proper spindle positioning and orientation are essential for asymmetric cell division and
require microtubule–actin filament (F-actin) interactions in many systems,. Such interactions�…

During morphogenesis, changes in the shapes of individual cells are harnessed to mold an
entire tissue. These changes in cell shapes require the coupled remodeling of the plasma�…

Cell surface expansion is a necessary part of cell shape change. One long-standing
hypothesis proposes that membrane for this expansion comes from the flattening out of cell�…

Regulated exocytosis is thought to occur either by “full fusion,” where the secretory vesicle
fuses with the plasma membrane (PM) via a fusion pore that then dilates until the secretory�…

We combine immunofluorescence and single-molecule fluorescence in situ hybridization
(smFISH), followed by automated image analysis, to quantify the concentration of nuclear�…

The actin filament (F-actin) cytoskeleton associates dynamically with the plasma membrane
and is thus ideally positioned to participate in endocytosis. Indeed, a wealth of genetic and�…

The stochastic kinetics of transcription is typically inferred from the distribution of RNA
numbers in individual cells. However, cellular RNA reflects additional processes�…

Compensatory endocytosis follows regulated exocytosis in cells ranging from eggs to
neurons, but the means by which it is accomplished are unclear. In Xenopus eggs�…

Cell-shape change demands cell-surface growth, but how growth is fueled and
choreographed is still debated. Here we use cellularization, the first complete cytokinetic�…

In early Drosophila embryos, several mitotic cycles proceed with aborted cytokinesis before
a modified cytokinesis, called cellularization, finally divides the syncytium into individual�…