The essence of successful mitosis is the generation of two genetically identical daughter
cells. This requires the assembly of a strictly bipolar mitotic apparatus that will ensure that all�…

Centrosome amplification (the presence of more than two centrosomes at mitosis) is
characteristic of many human cancers. Extra centrosomes can cause the assembly of�…

During mitosis in Ptk1 cells anaphase is not initiated until, on average, 23+/-1 min after the
last monooriented chromosome acquires a bipolar attachment to the spindle--an event that�…

To test the popular but unproven assumption that the metaphase-anaphase transition in
vertebrate somatic cells is subject to a checkpoint that monitors chromosome (ie�…

The abnormally high number of centrosomes found in many human tumor cells can lead
directly to aneuploidy and genomic instability through the formation of multipolar mitotic�…

Centrosomes were microsurgically removed from BSC-1 African green monkey kidney cells
before the completion of S phase. Karyoplasts (acentrosomal cells) entered and completed�…

How centrosome removal or perturbations of centrosomal proteins leads to G1 arrest in
untransformed mammalian cells has been a mystery. We use microsurgery and laser�…

Failure of cells to cleave at the end of mitosis is dangerous to the organism because it
immediately produces tetraploidy and centrosome amplification, which is thought to produce�…

The centrosome usually replicates in a semiconservative fashion, ie, new centrioles form in
association with preexisting “maternal” centrioles. De novo formation of centrioles has been�…

Exit from mitosis in animal cells is substantially delayed when spindle assembly is inhibited,
spindle bipolarity is disrupted, or when a monopolar spindle is formed. These observations�…