MYC (also known as c-MYC) overexpression or hyperactivation is one of the most common
drivers of human cancer. Despite intensive study, the MYC oncogene remains recalcitrant to�…

Myc is an oncogenic transcription factor frequently dysregulated in human cancer. To
identify pathways supporting the Myc oncogenic program, we used a genome-wide RNA�…

When the process of cell-fate determination is examined at single-cell resolution, it is often
observed that individual cells undergo different fates even when subject to identical�…

Transcription is a highly stochastic process. To infer transcription kinetics for a gene-of-
interest, researchers commonly compare the distribution of mRNA copy-number to the�…

We present a protocol for measuring the absolute number of mRNA molecules from a gene
of interest in individual, chemically fixed Escherichia coli cells. A set of fluorescently labeled�…

The ability of living cells to maintain an inheritable memory of their gene‐expression state is
key to cellular differentiation. Bacterial lysogeny serves as a simple paradigm for long‐term�…

The stochastic kinetics of transcription is typically inferred from the distribution of RNA
numbers in individual cells. However, cellular RNA reflects additional processes�…

Viral infection begins with the binding of a virus to a specific target on the surface of the host
cell, followed by viral genome delivery into the host and a continuation of the infection�…

Therapeutics that inhibit IL-6 at different points in its signaling pathway are in clinical use, yet
whether the immunological effects of these interventions differ based on their molecular�…

At diagnosis, most people with type 1 diabetes (T1D) produce measurable levels of
endogenous insulin, but the rate at which insulin secretion declines is heterogeneous. To�…