Fluorescence in situ hybridization (FISH), the assay of choice for localization of specific
nucleic acids sequences in native context, is a 20-year-old technology that has developed�…

The spatial regulation of protein translation is an efficient way to create functional and
structural asymmetries in cells. Recent research has furthered our understanding of how�…

The combined use of the new technologies of multiphoton-based intravital imaging, the
chemotaxis-mediated collection of invasive cells, and high sensitivity expression profiling�…

ASH1 mRNA localizes to the bud tip in Saccharomyces cerevisiae to establish asymmetry of
HO expression, important for mating type switching. To visualize real time localization of the�…

Fluorescence in situ hybridization (FISH) and digital imaging microscopy were modified to
allow detection of single RNA molecules. Oligodeoxynucleotide probes were synthesized�…

Specific labeling of biomolecules with bright fluorophores is the keystone of fluorescence
microscopy. Genetically encoded self-labeling tag proteins can be coupled to synthetic dyes�…

Proper execution of transcriptional programs is a key requirement of gene expression
regulation, demanding accurate control of timing and amplitude. How precisely the�…

It has not been possible to view the transcriptional activity of a single gene within a living
eukaryotic cell. It is therefore unclear how long and how frequently a gene is actively�…

We have developed an inducible system to visualize gene expression at the levels of DNA,
RNA and protein in living cells. The system is composed of a 200 copy transgene array�…

Localization of β-actin messenger RNA to sites of active actin polymerization modulates cell
migration during embryogenesis, differentiation and possibly carcinogenesis,,,,. This�…