User profiles for "author:Sep�lveda LA"

Leonardo A Sep�lveda

Postdoctoral Fellow, Harvard University
Verified email at fas.harvard.edu
Cited by 1297

General properties of transcriptional time series in Escherichia coli

L So, A Ghosh, C Zong, LA Sep�lveda, R Segev…�- Nature�…, 2011 - nature.com
Gene activity is described by the time series of discrete, stochastic mRNA production events.
This transcriptional time series shows intermittent, bursty behavior. One consequence of this�…

Measurement of gene regulation in individual cells reveals rapid switching between promoter states

LA Sep�lveda, H Xu, J Zhang, M Wang, I Golding�- Science, 2016 - science.org
In vivo mapping of transcription-factor binding to the transcriptional output of the regulated
gene is hindered by probabilistic promoter occupancy, the presence of multiple gene copies�…

Measuring mRNA copy number in individual Escherichia coli cells using single-molecule fluorescent in situ hybridization

SO Skinner, LA Sep�lveda, H Xu, I Golding�- Nature protocols, 2013 - nature.com
We present a protocol for measuring the absolute number of mRNA molecules from a gene
of interest in individual, chemically fixed Escherichia coli cells. A set of fluorescently labeled�…

Combining protein and mRNA quantification to decipher transcriptional regulation

H Xu, LA Sep�lveda, L Figard, AM Sokac, I Golding�- Nature methods, 2015 - nature.com
We combine immunofluorescence and single-molecule fluorescence in situ hybridization
(smFISH), followed by automated image analysis, to quantify the concentration of nuclear�…

Lysogen stability is determined by the frequency of activity bursts from the fate‐determining gene

C Zong, L So, LA Sep�lveda, SO Skinner…�- Molecular systems�…, 2010 - embopress.org
The ability of living cells to maintain an inheritable memory of their gene‐expression state is
key to cellular differentiation. Bacterial lysogeny serves as a simple paradigm for long‐term�…

[HTML][HTML] Single-virus tracking reveals a spatial receptor-dependent search mechanism

E Rothenberg, LA Sep�lveda, SO Skinner, L Zeng…�- Biophysical journal, 2011 - cell.com
Viral infection begins with the binding of a virus to a specific target on the surface of the host
cell, followed by viral genome delivery into the host and a continuation of the infection�…

The transcription fidelity factor GreA impedes DNA break repair

P Sivaramakrishnan, LA Sep�lveda, JA Halliday, J Liu…�- Nature, 2017 - nature.com
Homologous recombination repairs DNA double-strand breaks and must function even on
actively transcribed DNA. Because break repair prevents chromosome loss, the completion�…

Kinetics of basic dye adsorption onto sphagnum magellanicum peat

EG Contreras, BE Martinez…�- Adsorption Science�…, 2007 - journals.sagepub.com
The purpose of this study was to investigate the adsorption of two Basic dyes, ie Basic Blue
3 (BB3) and Basic Orange 2 (BO2), onto Sphagnum Magellanicum peat. The equilibrium�…

Valorization of agricultural wastes as dye adsorbents: characterization and adsorption isotherms

LA Sep�lveda, FA Cuevas…�- Environmental�…, 2015 - Taylor & Francis
The purpose of this work is to evaluate the valorization of agricultural waste, wheat straw
(WS) and corn cob leaves (CCLs) as textile dye adsorbents. Physico-chemical and�…

[HTML][HTML] The maternal-to-zygotic transition targets actin to promote robustness during morphogenesis

L Zheng, LA Sep�lveda, RC Lua, O Lichtarge…�- PLoS�…, 2013 - journals.plos.org
Robustness is a property built into biological systems to ensure stereotypical outcomes
despite fluctuating inputs from gene dosage, biochemical noise, and the environment�…