User profiles for "author:Sep�lveda LA"
![]() | Leonardo A Sep�lvedaPostdoctoral Fellow, Harvard University Verified email at fas.harvard.edu Cited by 1297 |
General properties of transcriptional time series in Escherichia coli
Gene activity is described by the time series of discrete, stochastic mRNA production events.
This transcriptional time series shows intermittent, bursty behavior. One consequence of this�…
This transcriptional time series shows intermittent, bursty behavior. One consequence of this�…
Measurement of gene regulation in individual cells reveals rapid switching between promoter states
In vivo mapping of transcription-factor binding to the transcriptional output of the regulated
gene is hindered by probabilistic promoter occupancy, the presence of multiple gene copies�…
gene is hindered by probabilistic promoter occupancy, the presence of multiple gene copies�…
Measuring mRNA copy number in individual Escherichia coli cells using single-molecule fluorescent in situ hybridization
We present a protocol for measuring the absolute number of mRNA molecules from a gene
of interest in individual, chemically fixed Escherichia coli cells. A set of fluorescently labeled�…
of interest in individual, chemically fixed Escherichia coli cells. A set of fluorescently labeled�…
Combining protein and mRNA quantification to decipher transcriptional regulation
We combine immunofluorescence and single-molecule fluorescence in situ hybridization
(smFISH), followed by automated image analysis, to quantify the concentration of nuclear�…
(smFISH), followed by automated image analysis, to quantify the concentration of nuclear�…
Lysogen stability is determined by the frequency of activity bursts from the fate‐determining gene
C Zong, L So, LA Sep�lveda, SO Skinner…�- Molecular systems�…, 2010 - embopress.org
The ability of living cells to maintain an inheritable memory of their gene‐expression state is
key to cellular differentiation. Bacterial lysogeny serves as a simple paradigm for long‐term�…
key to cellular differentiation. Bacterial lysogeny serves as a simple paradigm for long‐term�…
[HTML][HTML] Single-virus tracking reveals a spatial receptor-dependent search mechanism
E Rothenberg, LA Sep�lveda, SO Skinner, L Zeng…�- Biophysical journal, 2011 - cell.com
Viral infection begins with the binding of a virus to a specific target on the surface of the host
cell, followed by viral genome delivery into the host and a continuation of the infection�…
cell, followed by viral genome delivery into the host and a continuation of the infection�…
The transcription fidelity factor GreA impedes DNA break repair
P Sivaramakrishnan, LA Sep�lveda, JA Halliday, J Liu…�- Nature, 2017 - nature.com
Homologous recombination repairs DNA double-strand breaks and must function even on
actively transcribed DNA. Because break repair prevents chromosome loss, the completion�…
actively transcribed DNA. Because break repair prevents chromosome loss, the completion�…
Kinetics of basic dye adsorption onto sphagnum magellanicum peat
EG Contreras, BE Martinez…�- Adsorption Science�…, 2007 - journals.sagepub.com
The purpose of this study was to investigate the adsorption of two Basic dyes, ie Basic Blue
3 (BB3) and Basic Orange 2 (BO2), onto Sphagnum Magellanicum peat. The equilibrium�…
3 (BB3) and Basic Orange 2 (BO2), onto Sphagnum Magellanicum peat. The equilibrium�…
Valorization of agricultural wastes as dye adsorbents: characterization and adsorption isotherms
LA Sep�lveda, FA Cuevas…�- Environmental�…, 2015 - Taylor & Francis
The purpose of this work is to evaluate the valorization of agricultural waste, wheat straw
(WS) and corn cob leaves (CCLs) as textile dye adsorbents. Physico-chemical and�…
(WS) and corn cob leaves (CCLs) as textile dye adsorbents. Physico-chemical and�…
[HTML][HTML] The maternal-to-zygotic transition targets actin to promote robustness during morphogenesis
Robustness is a property built into biological systems to ensure stereotypical outcomes
despite fluctuating inputs from gene dosage, biochemical noise, and the environment�…
despite fluctuating inputs from gene dosage, biochemical noise, and the environment�…