In eukaryotes, the spindle-assembly checkpoint (SAC) is a ubiquitous safety device that
ensures the fidelity of chromosome segregation in mitosis. The SAC prevents chromosome�…

In this article, we review the dynamic nature of the filaments (microtubules) that make up the
labile fibers of the mitotic spindle and asters, we discuss the roles that assembly and�…

A replicated chromosome possesses two discrete, complex, dynamic, macromolecular
assemblies, known as kinetochores, that are positioned on opposite sides of the primary�…

We have developed video microscopy methods to visualize the assembly and disassembly
of individual microtubules at 33-ms intervals. Porcine brain tubulin, free of microtubule�…

Mitotic cells face the challenging tasks of linking kinetochores to growing and shortening
microtubules and actively regulating these dynamic attachments to produce accurate�…

We discovered that many proteins located in the kinetochore outer domain, but not the inner
core, are depleted from kinetochores and accumulate at spindle poles when ATP production�…

In mitotic cells, an error in chromosome segregation occurs when a chromosome is left near
the spindle equator after anaphase onset (lagging chromosome). In PtK1 cells, we found�…

Microtubules are involved in actin-based protrusion at the leading-edge lamellipodia of
migrating fibroblasts. Here we show that the growth of microtubules induced in fibroblasts by�…

Kinetochores are proteinaceous assemblies that mediate the interaction of chromosomes
with the mitotic spindle. The 180 kDa Ndc80 complex is a direct point of contact between�…

A single unattached kinetochore can delay anaphase onset in mitotic tissue culture cells
(Rieder, CL, A. Schultz, R. Cole, G. Sluder. 1994. J. Cell Biol. 127: 1301–1310)�…