Expression quantitative trait loci (eQTL) mapping studies have become a widely used tool
for identifying genetic variants that affect gene regulation. In these studies, expression levels�…

Changes in genetic regulation contribute to adaptations in natural populations and influence
susceptibility to human diseases. Despite their potential phenotypic importance, the�…

We describe a method for imaging individual mRNA molecules in fixed cells by probing
each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This�…

Little is known about broad patterns of variation and evolution of gene expression during
any developmental process. Here we investigate variation in genome-wide gene expression�…

The phenotypic differences between individual organisms can often be ascribed to
underlying genetic and environmental variation. However, even genetically identical�…

Metamorphosis is an integrated set of developmental processes controlled by a
transcriptional hierarchy that coordinates the action of hundreds of genes. In order to identify�…

Mutation is the ultimate source of biological diversity because it generates the variation that
fuels evolution. Gene expression is the first step by which an organism translates genetic�…

Identifying the properties of gene networks that influence their evolution is a fundamental
research goal. However, modes of evolution cannot be inferred solely from the distribution of�…

Recent experiments have revealed surprising behavior in the yeast galactose (GAL)
pathway, one of the preeminent systems for studying gene regulation. Under certain�…

We used a maskless photolithography method to produce DNA oligonucleotide microarrays
with unique probe sequences tiled throughout the genome of Drosophila melanogaster and�…