DNA methylation and chromatin modification are two global mechanisms that regulate gene
expression. Recent studies provide insight into the mechanism of transcriptional silencing by�…

Over the past few years, the long-standing idea that covalent modification of chromatin can
play a role in determining states of gene activity has been confirmed. Eukaryotic genes can�…

Reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) can be achieved
by viral-mediated transduction of defined transcription factors. Moving toward the eventual�…

Cytosine residues in the sequence 5′ CpG (cytosine–guanine) are often postsynthetically
methylated in animal genomes. CpG methylation is involved in long-term silencing of certain�…

Set1, the yeast histone H3-lysine 4 (H3-K4) methylase, is recruited by the Pol II elongation
machinery to a highly localized domain at the 5′ portion of active mRNA coding regions�…

Oct4 and Nanog are transcription factors required to maintain the pluripotency and self-
renewal of embryonic stem (ES) cells. Using the chromatin immunoprecipitation paired-end�…

Transcription factors (TFs) and their specific interactions with targets are crucial for
specifying gene-expression programs. To gain insights into the transcriptional regulatory�…

The ability to derive a whole-genome map of transcription-factor binding sites (TFBS) is
crucial for elucidating gene regulatory networks. Herein, we describe a robust approach that�…

Nanog, Sox2, and Oct4 are transcription factors all essential to maintaining the pluripotent
embryonic stem cell phenotype. Through a cooperative interaction, Sox2 and Oct4 have�…

ATP-dependent nucleosome remodeling and core histone acetylation and deacetylation
represent mechanisms to alter nucleosome structure. NuRD is a multisubunit complex�…