Fluorescence microscopy has become an essential tool in cell biology. This technique
allows researchers to visualize the dynamics of tissue, cells, individual organelles, and�…

For over a century, scientists have strived to understand the mechanisms that govern the
accurate segregation of chromosomes during mitosis. The most intriguing feature of this�…

Cell division is enacted by a microtubule-based, self-assembling macromolecular machine
known as the mitotic spindle. In 1986, Kirschner and Mitchison proposed that by undergoing�…

Background: In cells lacking centrosomes, the microtubule-organizing activity of the
centrosome is substituted for by the combined action of chromatin and molecular motors�…

Error-free chromosome segregation requires stable attachment of sister kinetochores to the
opposite spindle poles (amphitelic attachment). Exactly how amphitelic attachments are�…

The stable propagation of genetic material during cell division depends on the congression
of chromosomes to the spindle equator before the cell initiates anaphase. It is generally�…

During mitosis in Ptk1 cells anaphase is not initiated until, on average, 23+/-1 min after the
last monooriented chromosome acquires a bipolar attachment to the spindle--an event that�…

When centrosomes are destroyed during prophase by laser microsurgery, vertebrate
somatic cells form bipolar acentrosomal mitotic spindles (Khodjakov, A., RW Cole, BR�…

The centrosome usually replicates in a semiconservative fashion, ie, new centrioles form in
association with preexisting “maternal” centrioles. De novo formation of centrioles has been�…

Centrosome amplification is a common feature of many cancer cells, and it has been
previously proposed that centrosome amplification can drive genetic instability and so�…