We present a unified framework for extracting kinetic information from single-molecule
pulling experiments at constant force or constant pulling speed. Our procedure provides�…

Dynamic force spectroscopy probes the kinetic and thermodynamic properties of single
molecules and molecular assemblies. Here, we propose a simple procedure to extract�…

Dynamic force spectroscopy of single molecules is described by a model that predicts a
distribution of rupture forces, the corresponding mean rupture force, and variance, which are�…

Many essential processes in biology share a common fundamental step—establishing
physical contact between distant segments of DNA. How fast this step is accomplished sets�…

Single-molecule force experiments provide powerful new tools to explore biomolecular
interactions. Here, we describe a systematic procedure for extracting kinetic information from�…

Background Drosophila melanogaster females have two X chromosomes and two autosome
sets (XX; AA), while males have a single X chromosome and two autosome sets (X; AA)�…

During B lymphocyte development, immunoglobulin heavy-chain variable (VH), diversity
(DH), and joining (JH) segments assemble to generate a diverse antigen receptor repertoire�…

The folding and unfolding kinetics of single molecules, such as proteins or nucleic acids, can
be explored by mechanical pulling experiments. Determining intrinsic kinetic information, at�…

The capacity of biological macromolecules to act as exceedingly sophisticated and highly
efficient cellular machines–switches, assembly factors, pumps, or motors–is realized through�…

Single-molecule pulling experiments on the folding of biomolecules are usually interpreted
with one-dimensional models in which the dynamics occurs on the “pulling coordinate.”�…