Wnt signaling plays important roles in development and disease. The X-chromosomal
Porcupine homolog gene (Porcn) encodes an evolutionary conserved member of the�…

Transposable elements represent nearly half of mammalian genomes and are generally
described as parasites, or" junk DNA." The LINE1 retrotransposon is the most abundant�…

Spectral variants of green fluorescent protein are widely used in live samples for a broad
range of applications: from visualization of protein interactions, through following gene�…

Wnt/β-catenin signaling supports intestinal homeostasis by regulating proliferation in the
crypt. Multiple Wnts are expressed in Paneth cells as well as other intestinal epithelial and�…

Cultured pluripotent stem cells are a cornerstone of regenerative medicine owing to their
ability to give rise to all cell types of the body. Although pluripotent stem cells can be�…

During mammalian development, the first two lineages to be specified are the trophectoderm
(TE) and the inner cell mass (ICM). The Hippo pathway kinases Lats 1 and 2 (Lats1/2) and�…

In mice and humans the X-chromosomal porcupine homolog (Porcn) gene is required for the
acylation and secretion of all 19 Wnt ligands and thus represents a bottleneck for all Wnt�…

Within the first few days of life, the unipotent gametic genomes are rapidly reprogrammed to
support emergence of pluripotent cells in the early mammalian embryo. It is now appreciated�…

Mutational screens are an effective means used in the functional annotation of a genome.
We present a method for a mutational screen of the mouse X chromosome using gene trap�…

Ectoderm is one of the three classic germ layers in the early mouse embryo, with the
capacity to develop into both the central nervous system and epidermis. Because it is a�…