Chemical reactions contain an inherent element of randomness, which presents itself as
noise that interferes with cellular processes and communication. Here we discuss the ability�…

A central question in biology is whether variability between genetically identical cells
exposed to the same culture conditions is largely stochastic or deterministic. Using image�…

Fluorescence in situ hybridization (FISH) is widely used to obtain information about
transcript copy number and subcellular localization in single cells. However, current�…

The regulation of messenger RNA levels in mammalian cells can be achieved by the
modulation of synthesis and degradation rates. Metabolic RNA-labeling experiments in bulk�…

Most methods for single-cell transcriptome sequencing amplify the termini of polyadenylated
transcripts, capturing only a small fraction of the total cellular transcriptome. This precludes�…

Membrane-less organelles (MLOs) are liquid-like subcellular compartments that form
through phase separation of proteins and RNA. While their biophysical properties are�…

Cells sense the context in which they grow to adapt their phenotype and allow multicellular
patterning by mechanisms of autocrine and paracrine signalling,. However, patterns also�…

The epistatic interactions that underlie evolutionary constraint have mainly been studied for
constant external conditions. However, environmental changes may modulate epistasis and�…

Single-cell transcriptomics has recently emerged as one of the most promising tools for
understanding the diversity of the transcriptome among single cells. Image-based�…

Stress granules are phase-separated assemblies formed around RNAs. So far, the
techniques available to identify these RNAs are not suitable for single cells and small�…