Genetically engineered mouse models are commonly preferred for studying the human
disease due to genetic and pathophysiological similarities between mice and humans. In�…

The Cre/lox site-specific recombination system has emerged as an important tool for the
generation of conditional somatic mouse mutants. This method allows one to control gene�…

The cre‐loxP–mediated recombination system (the “cre‐loxP system”) is an integral
experimental tool for mammalian genetics and cell biology. Use of the system has greatly�…

The Cre/loxP system has become an important tool in designing postintegrational switch
mechanisms for transgenes in mice. The power and spectrum of application of this system�…

Molecular techniques now allow the design of precise genetic modifications in the mouse.
Not only can defined nucleotide changes be engineered into the genome of the mouse, but�…

Gene-targeted mice derived from embryonic stem cells are a useful tool to study gene
function during development. However, if the mutation is embryonic lethal and the gene is�…

In September 1998 the workshop for “Conditional Genome Alterations” in Cold Spring
Harbor had a simple but important take-home message:“Cre works”(Rossant and McMahon�…

The use of the Cre/loxP system has greatly empowered the field of gene targeting. Here we
describe the successful establishment of a novel knock‐in EGFP reporter mouse line to�…

The Cre‐lox system is an important tool for genetic manipulation. To promote integrative
reactions, two strategies using mutant lox sites have been developed. One is the left�…

The Cre/loxPsite-specific recombination system combined with embryonic stem cell-
mediated technologies has greatly expanded our capability to address normal and disease�…