[HTML][HTML] Next-generation sequencing: insights to advance clinical investigations of the microbiome

CR Wensel, JL Pluznick, SL Salzberg…�- The Journal of clinical�…, 2022 - Am Soc Clin Investig
Next-generation sequencing (NGS) technology has advanced our understanding of the
human microbiome by allowing for the discovery and characterization of unculturable�…

Best practices for analysing microbiomes

R Knight, A Vrbanac, BC Taylor, A Aksenov…�- Nature Reviews�…, 2018 - nature.com
Complex microbial communities shape the dynamics of various environments, ranging from
the mammalian gastrointestinal tract to the soil. Advances in DNA sequencing technologies�…

[HTML][HTML] Microbiome definition re-visited: old concepts and new challenges

G Berg, D Rybakova, D Fischer, T Cernava…�- Microbiome, 2020 - Springer
The field of microbiome research has evolved rapidly over the past few decades and has
become a topic of great scientific and public interest. As a result of this rapid growth in�…

[HTML][HTML] Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis

JS Johnson, DJ Spakowicz, BY Hong…�- Nature�…, 2019 - nature.com
The 16S rRNA gene has been a mainstay of sequence-based bacterial analysis for
decades. However, high-throughput sequencing of the full gene has only recently become a�…

Using MicrobiomeAnalyst for comprehensive statistical, functional, and meta-analysis of microbiome data

J Chong, P Liu, G Zhou, J Xia�- Nature protocols, 2020 - nature.com
MicrobiomeAnalyst is an easy-to-use, web-based platform for comprehensive analysis of
common data outputs generated from current microbiome studies. It enables researchers�…

MicrobiomeAnalyst 2.0: comprehensive statistical, functional and integrative analysis of microbiome data

Y Lu, G Zhou, J Ewald, Z Pang, T Shiri…�- Nucleic Acids�…, 2023 - academic.oup.com
Microbiome studies have become routine in biomedical, agricultural and environmental
sciences with diverse aims, including diversity profiling, functional characterization, and�…

[HTML][HTML] Simple statistical identification and removal of contaminant sequences in marker-gene and metagenomics data

NM Davis, DM Proctor, SP Holmes, DA Relman…�- Microbiome, 2018 - Springer
Background The accuracy of microbial community surveys based on marker-gene and
metagenomic sequencing (MGS) suffers from the presence of contaminants—DNA�…

[HTML][HTML] A communal catalogue reveals Earth's multiscale microbial diversity

LR Thompson, JG Sanders, D McDonald, A Amir…�- Nature, 2017 - nature.com
Our growing awareness of the microbial world's importance and diversity contrasts starkly
with our limited understanding of its fundamental structure. Despite recent advances in DNA�…

[HTML][HTML] Comparison between 16S rRNA and shotgun sequencing data for the taxonomic characterization of the gut microbiota

F Durazzi, C Sala, G Castellani, G Manfreda…�- Scientific reports, 2021 - nature.com
In this paper we compared taxonomic results obtained by metataxonomics (16S rRNA gene
sequencing) and metagenomics (whole shotgun metagenomic sequencing) to investigate�…

[HTML][HTML] Developmental trajectory of the healthy human gut microbiota during the first 5 years of life

J Roswall, LM Olsson, P Kovatcheva-Datchary…�- Cell host &�…, 2021 - cell.com
The gut is inhabited by a densely populated ecosystem, the gut microbiota, that is
established at birth. However, the succession by which different bacteria are incorporated�…