Anaerobic oxidation of methane (AOM) is a biological process that plays an important role in reducing the CH₄ emissions from a wide range of ecosystems. Arctic and sub-Arctic lakes are recognized as significant contributors to global methane (CH₄) emission, since CH₄ production is increasing as permafrost thaws and provides fuels for methanogenesis. Methanotrophy, including AOM, is critical to reducing CH₄ emissions. The identity, activity, and metabolic processes of anaerobic methane oxidizers are poorly understood, yet this information is critical to understanding CH₄ cycling and ultimately to predicting future CH₄ emissions. This study sought to identify the microorganisms involved in AOM in sub-Arctic lake sediments using DNA- and phospholipid-fatty acid (PLFA)- based stable isotope probing. Results indicated that aerobic methanotrophs belonging to the genus Methylobacter assimilate carbon from CH₄, either directly or indirectly. Other organisms that were found, in minor proportions, to assimilate CH₄-derived carbon were methylotrophs and iron reducers, which might indicate the flow of CH₄-derived carbon from anaerobic methanotrophs into the broader microbial community. While various other taxa have been reported in the literature to anaerobically oxidize methane in various environments (e.g. ANME-type archaea and Methylomirabilis Oxyfera), this report directly suggest that Methylobacter can perform this function, expanding our understanding of CH₄ oxidation in anaerobic lake sediments.