Methods: Three mosquito vectors belonging to the genus Aedes, Anopheles and Culex were
microinjected at different developmental stages of their life cycle viz., egg, larvae, pupae …

Due to the recent interest in the development of novel genetic methods to control insect vectors
of disease, a great deal of research has focused on the genetic manipulation of the insect …

… Morris AC (1997) In: Crampton JM, Beard CB, Louis C (eds) Microinjection of mosquito
embryos. In molecular biology of insect disease vectors: a methods manual. Chapman & Hall, pp …

Genetic strategies that reduce or block pathogen transmission by mosquitoes are being
investigated as a means to augment current control measures. Strategies of vector suppression …

Genetic transformation is an important technology that provides unique opportunities to find,
isolate, and analyze genes, as well as to create organisms with unique functional …

The piggyBac transposable element was tested for transposition activity in plasmid-based
excision and inter-plasmid transposition assays to determine if this element would function in …

Using a plasmid-based transpositional recombination assay in vivo, we have demonstrated
that Hermes, a short inverted repeat type transposable element from Musca domestica, can …

… Microinjection of Mosquito Embryos Here, we describe the system which we have developed
in our own laboratory for transformation of the mosquito Aedes aegypti. However, similar …

A Hermes-based transposable element transformation system incorporating an enhanced
green fluorescent protein (EGFP) marker was used to produce two transgenic lines of Culex …

The process of transferring Wolbachia from one species to another to establish a stable,
maternally inherited infection in the target species is known as transinfection. The success of …