Field-deployable viral diagnostics using CRISPR-Cas13
Mitigating global infectious disease requires diagnostic tools that are sensitive, specific, and
rapidly field deployable. In this study, we demonstrate that the Cas13-based SHERLOCK�…
rapidly field deployable. In this study, we demonstrate that the Cas13-based SHERLOCK�…
Nucleic acid detection with CRISPR-Cas13a/C2c2
Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen
detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting clustered�…
detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting clustered�…
Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6
Rapid detection of nucleic acids is integral for clinical diagnostics and biotechnological
applications. We recently developed a platform termed SHERLOCK (specific high-sensitivity�…
applications. We recently developed a platform termed SHERLOCK (specific high-sensitivity�…
[HTML][HTML] Rapid, low-cost detection of Zika virus using programmable biomolecular components
The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be
rapidly developed for distribution and use in pandemic regions. Here, we report a pipeline�…
rapidly developed for distribution and use in pandemic regions. Here, we report a pipeline�…
CRISPR-based surveillance for COVID-19 using genomically-comprehensive machine learning design
HC Metsky, CA Freije, TSF Kosoko-Thoroddsen…�- BioRxiv, 2020 - biorxiv.org
The emergence and outbreak of SARS-CoV-2, the causative agent of COVID-19, has rapidly
become a global concern and has highlighted the need for fast, sensitive, and specific tools�…
become a global concern and has highlighted the need for fast, sensitive, and specific tools�…
[HTML][HTML] Massively multiplexed nucleic acid detection with Cas13
The great majority of globally circulating pathogens go undetected, undermining patient care
and hindering outbreak preparedness and response. To enable routine surveillance and�…
and hindering outbreak preparedness and response. To enable routine surveillance and�…
[PDF][PDF] CRISPR-based COVID-19 surveillance using a genomically-comprehensive machine learning approach
The emergence and outbreak of SARS-CoV-2, the causative agent of COVID-19, has rapidly
become a global concern and has highlighted the need for fast, sensitive, and specific�…
become a global concern and has highlighted the need for fast, sensitive, and specific�…
[HTML][HTML] Cutting-edge infectious disease diagnostics with CRISPR
C Chiu�- Cell host & microbe, 2018 - cell.com
Three recent Science articles (Chen et al., 2018; Gootenberg et al., 2018; Myhrvold et al.,
2018) describe the use of CRISPR-Cas technology to develop point-of-care diagnostics that�…
2018) describe the use of CRISPR-Cas technology to develop point-of-care diagnostics that�…
[HTML][HTML] An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing
Extensive testing is essential to break the transmission of SARS-CoV-2, which causes the
ongoing COVID-19 pandemic. Here, we present a CRISPR-based diagnostic assay that is�…
ongoing COVID-19 pandemic. Here, we present a CRISPR-based diagnostic assay that is�…
[HTML][HTML] Multiplexed CRISPR-based microfluidic platform for clinical testing of respiratory viruses and identification of SARS-CoV-2 variants
Abstract The coronavirus disease 2019 (COVID-19) pandemic has demonstrated a clear
need for high-throughput, multiplexed and sensitive assays for detecting severe acute�…
need for high-throughput, multiplexed and sensitive assays for detecting severe acute�…