The assessment of collagen content in tissues is important in biomedical research, since this
protein is altered in numerous diseases. Hydroxyproline and Sirius red based assays are�…

Collagen is the major extracellular protein in mammals. Accurate quantification of collagen
is essential in the biomaterials (eg, reproducible collagen scaffold fabrication), drug�…

Collagen contains the unique imino acid hydroxyproline (HyPro), which is involved in the
stabilization of this triple helical molecule. The concentration of HyPro is customarily used to�…

Tissue fibrosis is characterized by chronic fibroblast activation and consequently excessive
accumulation of collagen-rich extracellular matrix. In vitro microplate-based assays are�…

By using a picrosirius dye, sensitive and specific staining of collagens plated in microtiter
wells was achieved. The range of detection was from 0.5 to 20 μg. Human collagen types I�…

A method for the determination and quantification of collagen types (IV) using sample
pretreatment has been developed. This work is a continuation of our previous work dealing�…

A method to estimate collagen concentration is described. It is based on the ability of the dye
Sirius Red F3BA to quantitatively precipitate collagen in acetic acid solution. Collagen�…

A method for the determination and quantification of collagen types I–V in rat tissues has
been developed. This method is based on collagen fragmentation by cyanogen bromide�…

Sensitive and selective assay of collagen is of substantial importance to the diagnostic study
of health-and aging-related failures. In this paper, we describe a highly specific and�…

A method to quantitate collagen in solution is described. It is based on binding of the dye
Sirius Supra red F3BA by collagen followed by elution and estimation of the bound dye in a�…