Background Current imaging methods such as Magnetic Resonance Imaging (MRI),
Confocal microscopy, Electron Microscopy (EM) or Selective Plane Illumination Microscopy�…

Understanding the structure–function relationship of cells and organelles in their natural
context requires multidimensional imaging. As techniques for multimodal 3-D imaging have�…

Abstract Open-Source 3D Visualization-Assisted Analysis (Vaa3D) is a software platform for
the visualization and analysis of large-scale multidimensional images. In this protocol we�…

Mayavi is a general purpose, open source 3D scientific visualization package that is tightly
integrated with the rich ecosystem of Python scientific packages. Mayavi provides a�…

Confocal microscopy is widely used in neurobiology for studying the three-dimensional
structure of the nervous system. Confocal image data are often multi-channel, with each�…

A comprehensive software package, called ANALYZE, which permits detailed investigation
and evaluation of 3D and 4D biomedical images is described. This software can be used�…

In a wide range of biological studies, it is highly desirable to visualize and analyze three-
dimensional (3D) microscopic images. In this primer, we first introduce several major�…

Laboratory for Optical and Computational Instrumentation, University of Wisconsin at
Madison, Madison, WI, USA sional approaches with an emphasis on 4-D data and present�…

Confocal and two-photon fluorescence microscopy have advanced the exploration of
complex, three-dimensional biological structures at submicron resolution. We have�…

There are many medical image processing software tools available for research and
diagnosis purposes. However, most of these tools are available only as local applications�…