Here we use the clustered, regularly interspaced, short palindromic repeats (CRISPR)–
associated Cas9 endonuclease complexed with dual-RNAs to introduce precise mutations�…

Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen
detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting clustered�…

Index decomposition methodology was a technique first used in the late 1970s to study the
impact of changes in product mix on industrial energy demand. A survey in 1995 listed a�…

Systematic interrogation of gene function requires the ability to perturb gene expression in a
robust and generalizable manner. Here we describe structure-guided engineering of a�…

Coagulopathy in Critical Illness with Covid-19 The authors describe a 69-year-old man with
Covid-19 diagnosed in January 2020 in Wuhan, China, along with two other critically ill�…

BRCA1 and BRCA2 are often mutated in familial breast and ovarian cancer. Both tumor
suppressors play key roles in the DNA-damage response [1, 2]. However, it remains unclear�…

Mitigating global infectious disease requires diagnostic tools that are sensitive, specific, and
rapidly field deployable. In this study, we demonstrate that the Cas13-based SHERLOCK�…

Synchronized oscillations and inhibitory interneurons have important and interconnected
roles within cortical microcircuits. In particular, interneurons defined by the fast-spiking�…

Abstract Cortical gamma oscillations (20-80 Hz) predict increases in focused attention, and
failure in gamma regulation is a hallmark of neurological and psychiatric disease. Current�…

Frame-disrupting mutations in the DMD gene, encoding dystrophin, compromise myofiber
integrity and drive muscle deterioration in Duchenne muscular dystrophy (DMD). Removing�…