SM-Youtube

Year	Number of Results
2004	1
2005	1
2006	3
2007	1
2008	67
2009	234
2010	278
2011	334
2012	295
2013	256
2014	311
2015	320
2016	318
2017	298
2018	286
2019	365
2020	364
2021	305
2022	59
2023	27
2024	64

1

MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems.

Sakuma T, Nakade S, Sakane Y, Suzuki KT, Yamamoto T. Sakuma T, et al. Nat Protoc. 2016 Jan;11(1):118-33. doi: 10.1038/nprot.2015.140. Epub 2015 Dec 17. Nat Protoc. 2016. PMID: 26678082

2

Knock-In of a Large Reporter Gene via the High-Throughput Microinjection of the CRISPR/Cas9 System.

Chen S, Jiao Y, Pan F, Guan Z, Cheng SH, Sun D. Chen S, et al. IEEE Trans Biomed Eng. 2022 Aug;69(8):2524-2532. doi: 10.1109/TBME.2022.3149530. Epub 2022 Jul 18. IEEE Trans Biomed Eng. 2022. PMID: 35133958

3

Reporter gene knock-in into Marc-145 cells using CRISPR/Cas9-mediated homologous recombination.

Chang Y, Shao J, Gao Y, Liu W, Gao Z, Hu Y, Chang H. Chang Y, et al. Biotechnol Lett. 2020 Aug;42(8):1317-1325. doi: 10.1007/s10529-020-02860-x. Epub 2020 Mar 17. Biotechnol Lett. 2020. PMID: 32185620

4

Gene Knock-Ins in Drosophila Using Homology-Independent Insertion of Universal Donor Plasmids.

Bosch JA, Colbeth R, Zirin J, Perrimon N. Bosch JA, et al. Genetics. 2020 Jan;214(1):75-89. doi: 10.1534/genetics.119.302819. Epub 2019 Nov 4. Genetics. 2020. PMID: 31685521 Free PMC article.

5

Rosa26-targeted sheep gene knock-in via CRISPR-Cas9 system.

Wu M, Wei C, Lian Z, Liu R, Zhu C, Wang H, Cao J, Shen Y, Zhao F, Zhang L, Mu Z, Wang Y, Wang X, Du L, Wang C. Wu M, et al. Sci Rep. 2016 Apr 11;6:24360. doi: 10.1038/srep24360. Sci Rep. 2016. PMID: 27063570 Free PMC article.

6

Cross-tissue organization of the fibroblast lineage.

Buechler MB, Pradhan RN, Krishnamurty AT, Cox C, Calviello AK, Wang AW, Yang YA, Tam L, Caothien R, Roose-Girma M, Modrusan Z, Arron JR, Bourgon R, Müller S, Turley SJ. Buechler MB, et al. Nature. 2021 May;593(7860):575-579. doi: 10.1038/s41586-021-03549-5. Epub 2021 May 12. Nature. 2021. PMID: 33981032

7

Recent advances in CRISPR/Cas9-mediated knock-ins in mammalian cells.

Banan M. Banan M. J Biotechnol. 2020 Jan 20;308:1-9. doi: 10.1016/j.jbiotec.2019.11.010. Epub 2019 Nov 18. J Biotechnol. 2020. PMID: 31751596 Review.

8

A highly efficient transgene knock-in technology in clinically relevant cell types.

Allen AG, Khan SQ, Margulies CM, Viswanathan R, Lele S, Blaha L, Scott SN, Izzo KM, Gerew A, Pattali R, Cochran NR, Holland CS, Zhao AH, Sherman SE, Jaskolka MC, Wu M, Wilson AC, Sun X, Ciulla DM, Zhang D, Nelson JD, Zhang P, Mazzucato P, Huang Y, Giannoukos G, Marco E, Nehil M, Follit JA, Chang KH, Shearman MS, Wilson CJ, Zuris JA. Allen AG, et al. Nat Biotechnol. 2024 Mar;42(3):458-469. doi: 10.1038/s41587-023-01779-8. Epub 2023 May 1. Nat Biotechnol. 2024. PMID: 37127662

9

Generation of Tfap2c-T2A-tdTomato knock-in reporter rats via adeno-associated virus-mediated efficient gene targeting.

Oikawa M, Nagae M, Mizuno N, Iwatsuki K, Yoshida F, Inoue N, Uenoyama Y, Tsukamura H, Nakauchi H, Hirabayashi M, Kobayashi T. Oikawa M, et al. Mol Reprod Dev. 2022 Mar;89(3):129-132. doi: 10.1002/mrd.23562. Epub 2022 Feb 15. Mol Reprod Dev. 2022. PMID: 35170139

10

Harnessing endogenous repair mechanisms for targeted gene knock-in of bovine embryos.

Owen JR, Hennig SL, McNabb BR, Lin JC, Young AE, Murray JD, Ross PJ, Van Eenennaam AL. Owen JR, et al. Sci Rep. 2020 Sep 29;10(1):16031. doi: 10.1038/s41598-020-72902-x. Sci Rep. 2020. PMID: 32994506 Free PMC article.

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