Fans may experience significant upset and distress when a television series ends. However, grief and loss reactions to the end of a fictional series have seldom been investigated. It is likely that the degree to which such reactions are felt is influenced by viewing motives (e.g., pleasure, meaning making), connection to the series and its fan community, relationships formed with characters, including parasocial bonds and experiences of empathy, and tendency to engage with others' perspectives and emotions, including fictional characters. The purpose of this study was to examine predictors of fans' grief and loss reactions to the end of the television series Neighbours, which aired from 1985 to 2022. Fans (n = 1289) completed an online survey shortly after the screening of the final episode. The survey measured grief emotions and cognitions, acceptance that the series had ended, distress at the loss of a parasocial relationship with a favourite character, feelings of closure, and expressions of gratitude for the series. Predictors of these grief and loss reactions examined in the survey were viewing motives, fan identity, strength of a parasocial relationship formed with a favourite character, empathy towards that character, and tendency to take others' perspectives, experience empathic concern and personal distress, and tendency towards engagement with fictional characters. Greater grief and loss reactions were experienced by fans whose motives for watching involved being entertained and exposed to different lifestyles, who felt a stronger fan connection to the series, and who formed stronger parasocial empathic relationships with their favourite character. Factors such as gender, age, and empathic tendencies predicted various types of grief reactions. Understanding fan experiences when a long-running series ends advances theory and research on viewer parasocial relationships and engagement with media, as well as providing evidence that the loss of a series or favourite character can be viewed as a type of grief experience.