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. 2024 May 24:21:200290.
doi: 10.1016/j.ijcrp.2024.200290. eCollection 2024 Jun.

Renal denervation ameliorated salt-induced hypertension by improving cardiac work, cardiac enzyme and oxidative balance in Sprague-Dawley rats

Abdullahi Adejare  1 , Ahmed Oloyo  1 , Yusuf Dahud  1 , Morufat Adeshina  1 , Abiola Agbaje  1 , Clinton Ejim  1 , Khadijah Ismail-Badmus  1 , Smith Jaja  1
Affiliations

Renal denervation ameliorated salt-induced hypertension by improving cardiac work, cardiac enzyme and oxidative balance in Sprague-Dawley rats

Abdullahi Adejare et al. Int J Cardiol Cardiovasc Risk Prev. .

Abstract

Background: Hypertension is associated with cardiovascular dysfunction, dysregulation of the antioxidant system and alteration of the level of some enzymes in the metabolic pathway. The possible modulatory effect of acute renal denervation (ARD) on cardiovascular function and the antioxidant system is still a subject of intense debate. This study sought to ascertain the ameliorative effects of ARD on cardiovascular parameters, antioxidant system, creatine kinase and lactate dehydrogenase levels.

Methods: Thirty-six Sprague-Dawley rats (5-6 weeks old) were divided into 6 groups of 6 animals each consisting of Normal Salt, High Salt, Normal Salt + Sham Denervation, High Salt + Sham Denervation, Normal Salt + Renal Denervation and High Salt + Renal Denervation. Induction of hypertension with 8 % salt in the diet lasted for 8 weeks. Renal or Sham denervation was thereafter done on selected groups. At the end of the experimental period, cardiovascular parameters, plasma antioxidant status, plasma creatine kinase (CK) and lactate dehydrogenase (LDH) levels were assessed. Significance level was set at p < 0.05.

Results: Salt-loading significantly increased systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial blood pressure (MABP), rate pressure product (RPP) while reducing superoxide dismutase (SOD), reduced glutathione (GSH) and catalase (CAT). Acute renal denervation significantly (p < 0.0001) reduced SBP, DBP, MABP, RPP, LDH and norepinephrine level while increasing SOD, GSH and CAT. ARD did not significantly alter CK level.

Conclusion: Acute renal denervation, by reducing sympathetic activity, ameliorates cardiovascular and antioxidant functions as well as reduces LDH level without significantly altering CK level in salt-induced hypertension.

Keywords: Acute renal denervation; Antioxidant enzymes; Cardiovascular parameters; Creatine kinase; Lactate dehydrogenase.

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Conflict of interest statement

None.

Figures

Fig. 1
Fig. 1
Creatine kinase level across the groups.
Fig. 2
Fig. 2
Lactate dehydrogenase level across the groups Bars represent Mean ± SEM. Significant (* = p < 0.05 vs HS diet group).
Fig. 3
Fig. 3
Norepinephrine level across the groups. Bars represent Mean ± SEM. Significant (* = p < 0.05 vs HS diet group).
See this image and copyright information in PMC

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References

    1. Mancia G., Laurent S., Agabiti-Rosei E. Reappraisal of European guidelines on hypertension management: a European society of hypertension task force document. J. Hypertens. 2013;27:2121–2158. - PubMed
    1. Zambon A., Arfè A., Corrao G., Zanchetti A. Relationships of different types of event to cardiovascular death in trials of antihypertensive treatment: an aid to definition of total cardiovascular disease risk in hypertension. J. Hypertens. 2014;32(3):495–508. - PubMed
    1. Adejare A.A., Oloyo A.K., Ishola I.O., Busari A.A., Ismail-Badmus K.B., Abdulrazaq M.M. Black seed (nigella sativa) oil restores smoke or nicotine-induced vascular impairment via improvement in endothelium-dependent relaxation: role of nitric oxide synthase and voltage-sensitive potassium channe. J. Afr. Ass. Physiol. Sci. 2023;11(1):1–16.
    1. Kearney P.M., Whelton M., Reynolds K., et al. Global burden of hypertension: analysis of worldwide data. Lancet. 2005;365:217–223. - PubMed
    1. Vasdev S., Gill V. The antihypertensive effect of arginine. Int. J. Angiol. 2008;17(1):7–22. - PMC - PubMed