Identification of plasma and urinary inflammatory markers in severe knee osteoarthritis: Relations with synovial fluid markers
- PMID: 38773579
- PMCID: PMC11106897
- DOI: 10.1186/s43019-024-00223-8
Identification of plasma and urinary inflammatory markers in severe knee osteoarthritis: Relations with synovial fluid markers
Abstract
Background: This study aimed to identify plasma and urinary cytokines as potential biomarkers for severe knee osteoarthritis (OA). It also investigated associations between these cytokines and cartilage markers, as well as their connections with synovial fluid (SF) markers.
Methods: Samples of plasma, urine, and SF were obtained from patients (n = 40) undergoing total knee arthroplasty (TKA) or unicompartmental knee arthroplasty (UKA) due to severe knee OA. Control samples of plasma and urine were collected from non-OA individuals (n = 15). We used a Luminex immunoassay for the simultaneous measurement of 19 cytokines, MMP-1, and MMP-3 levels. COMP, CTX-II, and hyaluronan (HA) levels were quantified using enzyme-linked immunosorbent assay (ELISA) kits. Receiver operating characteristic (ROC) curves were utilized to analyze each biomarker's performance. Correlations among these biomarkers were evaluated via Spearman's correlation.
Results: The levels of plasma (p)CCL11, pCXCL16, pIL-8, pIL-15, pHA, urinary (u)CCL2, uCCL11, uCCL19, uCXCL16, uIL-1β, uIL-6, uIL-8, uIL-12p70, uIL-15, uIL-33, uMMP-3, uHA, uCTX-II, and uCOMP were significantly elevated in individuals with severe knee OA. Notably, specific correlations were observed between the plasma/urine biomarkers and SF biomarkers: pCCL11 with sfHA (r = 0.56) and sfTNF-α (r = 0.58), pIL-15 with sfCCL19 (r = 0.43) and sfCCL20 (r = 0.44), and uCCL19 with sfCCL11 (r = 0.45) and sfIL-33 (r = 0.51). Positive correlations were also observed between uCCL11 and its corresponding sfCCL11(r = 0.49), as well as between sfCCL11 and other cytokines, namely sfCCL4, sfCCL19, sfCCL20, sfIL-33, and sfTNF-α (r = 0.46-0.63).
Conclusion: This study provides an extensive profile of systemic inflammatory mediators in plasma of knee OA and identified four inflammatory markers (pCCL11, pIL-15, uCCL11, and uCCL19) reflecting joint inflammation.
Keywords: Cytokine; Osteoarthritis; Plasma; Synovial fluid; Urine.
© 2024. The Author(s).
Conflict of interest statement
All authors declare no competing interest.
Figures
![Fig. 1](https://cdn.statically.io/img/www.ncbi.nlm.nih.gov/pmc/articles/instance/11106897/bin/43019_2024_223_Fig1_HTML.gif)
![Fig. 2](https://cdn.statically.io/img/www.ncbi.nlm.nih.gov/pmc/articles/instance/11106897/bin/43019_2024_223_Fig2_HTML.gif)
Similar articles
-
Synovial fluid biomarkers associated with osteoarthritis severity reflect macrophage and neutrophil related inflammation.Arthritis Res Ther. 2019 Jun 13;21(1):146. doi: 10.1186/s13075-019-1923-x. Arthritis Res Ther. 2019. PMID: 31196179 Free PMC article. Clinical Trial.
-
Synovial fluid levels of bradykinin correlate with biochemical markers for cartilage degradation and inflammation in knee osteoarthritis.Osteoarthritis Cartilage. 2013 Nov;21(11):1774-80. doi: 10.1016/j.joca.2013.08.014. Epub 2013 Aug 11. Osteoarthritis Cartilage. 2013. PMID: 23942063
-
Cross sectional evaluation of biochemical markers of bone, cartilage, and synovial tissue metabolism in patients with knee osteoarthritis: relations with disease activity and joint damage.Ann Rheum Dis. 2001 Jun;60(6):619-26. doi: 10.1136/ard.60.6.619. Ann Rheum Dis. 2001. PMID: 11350852 Free PMC article.
-
Interleukin-7 levels in synovial fluid increase with age and MMP-1 levels decrease with progression of osteoarthritis.Acta Orthop. 2012 Feb;83(1):59-64. doi: 10.3109/17453674.2011.645195. Epub 2011 Dec 29. Acta Orthop. 2012. PMID: 22206448 Free PMC article.
-
Elevated plasma and synovial fluid interleukin-8 and interleukin-18 may be associated with the pathogenesis of knee osteoarthritis.Knee. 2020 Jan;27(1):26-35. doi: 10.1016/j.knee.2019.10.028. Epub 2020 Jan 6. Knee. 2020. PMID: 31917106
References
-
- Meehan RT, Regan EA, Hoffman ED, Wolf ML, Gill MT, Crooks JL, et al. Synovial fluid cytokines, chemokines and MMP levels in osteoarthritis patients with knee pain display a profile similar to many rheumatoid arthritis patients. J Clin Med. 2021;10(21):5027. doi: 10.3390/jcm10215027. - DOI - PMC - PubMed
Grants and funding
LinkOut - more resources
Full Text Sources
Miscellaneous