Spatial transition tensor of single cells
- PMID: 38755322
- PMCID: PMC11166574
- DOI: 10.1038/s41592-024-02266-x
Spatial transition tensor of single cells
Abstract
Spatial transcriptomics and messenger RNA splicing encode extensive spatiotemporal information for cell states and transitions. The current lineage-inference methods either lack spatial dynamics for state transition or cannot capture different dynamics associated with multiple cell states and transition paths. Here we present spatial transition tensor (STT), a method that uses messenger RNA splicing and spatial transcriptomes through a multiscale dynamical model to characterize multistability in space. By learning a four-dimensional transition tensor and spatial-constrained random walk, STT reconstructs cell-state-specific dynamics and spatial state transitions via both short-time local tensor streamlines between cells and long-time transition paths among attractors. Benchmarking and applications of STT on several transcriptome datasets via multiple technologies on epithelial-mesenchymal transitions, blood development, spatially resolved mouse brain and chicken heart development, indicate STT's capability in recovering cell-state-specific dynamics and their associated genes not seen using existing methods. Overall, STT provides a consistent multiscale description of single-cell transcriptome data across multiple spatiotemporal scales.
© 2024. The Author(s).
Conflict of interest statement
The authors declare no competing interests.
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Grants and funding
- R01 AR079150/AR/NIAMS NIH HHS/United States
- R01AR079150/Foundation for the National Institutes of Health (Foundation for the National Institutes of Health, Inc.)
- U01 AR073159/AR/NIAMS NIH HHS/United States
- MCB2028424/National Science Foundation (NSF)
- U01AR073159/Foundation for the National Institutes of Health (Foundation for the National Institutes of Health, Inc.)
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