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. 2024 May;106(3):171-180.
doi: 10.1002/cyto.b.22178. Epub 2024 May 2.

The role of CD20+ T cells: Insights in human peripheral blood

Aryane Cruz Oliveira Pinho  1   2   3 , Pedro Barbosa  1   2   4 , Maria João Pereira  5 , Artur Paiva  2   6   7   8   9 , Eugenia Carvalho  1   2   4   10 , Paula Laranjeira  1   2   6   7   8
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The role of CD20+ T cells: Insights in human peripheral blood

Aryane Cruz Oliveira Pinho et al. Cytometry B Clin Cytom. 2024 May.

Abstract

CD20+ T cells constitute a small subset of T cells. These are found among CD4+, CD8+, CD4+CD8+, CD4-CD8- T, and TCRγδ+ T cells, and have been poorly characterized. The aim of this study was to characterize peripheral blood (PB) CD20+ T cells and compare them to their PB CD20- T cell counterparts. PB from 17 healthy individuals was collected. The distribution of CD20+ T cells among maturation-associated T cells compartments (naïve, central memory, transitional memory, effector memory, and effector T cells), their polarization, activation status, and expression of immune-regulatory proteins were evaluated by flow cytometry. Their function was also assessed, by measuring IFN-γ, TNF-α, and IL-17 production. Compared with CD20- T cells, CD20+ T cells represent a higher proportion of transitional memory cells. Furthermore, CD20+ T cells display a proinflammatory phenotype, characterized by the expansion of Th1, Th1/17, and Tc1 cell subsets , associated to a high expression of activation (CD25) and exhaustion (PD-1) markers. In addition, the simultaneous production of the proinflammatory cytokines IFN-γ, TNF-α, and IL-17 was also detected in CD4+CD20+ T cells. Our results show that CD20+ T cells are phenotypically and functionally different from CD20- T cells, suggesting that these cells are a distinct subset of T cells.

Keywords: CD20+ T cells; activation and exhaustion status; cytokines; flow cytometry; functional compartments; polarization.

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