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Review
. 2024 Apr 12;40(5):159.
doi: 10.1007/s11274-024-03951-x.

Microbial chassis design and engineering for production of gamma-aminobutyric acid

Jianli Wang  1   2   3 , Wenjian Ma  1   2   4 , Jingwen Zhou  1   4 , Xiaoyuan Wang  5   6   7 , Lei Zhao  8
Affiliations
Review

Microbial chassis design and engineering for production of gamma-aminobutyric acid

Jianli Wang et al. World J Microbiol Biotechnol. .

Abstract

Gamma-aminobutyric acid (GABA) is a non-protein amino acid which is widely applied in agriculture and pharmaceutical additive industries. GABA is synthesized from glutamate through irreversible α-decarboxylation by glutamate decarboxylase. Recently, microbial synthesis has become an inevitable trend to produce GABA due to its sustainable characteristics. Therefore, reasonable microbial platform design and metabolic engineering strategies for improving production of GABA are arousing a considerable attraction. The strategies concentrate on microbial platform optimization, fermentation process optimization, rational metabolic engineering as key metabolic pathway modification, promoter optimization, site-directed mutagenesis, modular transporter engineering, and dynamic switch systems application. In this review, the microbial producers for GABA were summarized, including lactic acid bacteria, Corynebacterium glutamicum, and Escherichia coli, as well as the efficient strategies for optimizing them to improve the production of GABA.

Keywords: Corynebacterium glutamicum; Escherichia coli; Gamma-aminobutyric acid (GABA); Lactic acid bacteria; Metabolic engineering; Microbial chassis; Synthetic biology.

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References

    1. Baritugo KA, Kim HT, David Y, Khang TU, Hyun SM, Kang KH, Yu JH, Choi JH, Song JJ, Joo JC, Park SJ (2018) Enhanced production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum strains from empty fruit bunch biosugar solution. Microb Cell Fact 17(1):129. https://doi.org/10.1186/s12934-018-0977-9 - DOI
    1. Cataldo PG, Villegas JM, Savoy de Giori G, Saavedra L, Hebert EM (2020) Enhancement of gamma-aminobutyric acid (GABA) production by Lactobacillus brevis CRL 2013 based on carbohydrate fermentation. Int J Food Microbiol 333:108792. https://doi.org/10.1016/j.ijfoodmicro.2020.108792 - DOI
    1. Cho YR, Chang JY, Chang HC (2007) Production of gamma-aminobutyric acid (GABA) by Lactobacillus buchneri isolated from kimchi and its neuroprotective effect on neuronal cells. J Microbiol Biotechnol 17(1):104–109
    1. Cho JS, Choi KR, Prabowo CPS, Shin JH, Yang D, Jang J, Lee SY (2017) CRISPR/Cas9-coupled recombineering for metabolic engineering of Corynebacterium glutamicum. Metab Eng 42:157–167. https://doi.org/10.1016/j.ymben.2017.06.010 - DOI
    1. Choi JW, Yim SS, Lee SH, Kang TJ, Park SJ, Jeong KJ (2015) Enhanced production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum by expressing glutamate decarboxylase active in expanded pH range. Microb Cell Fact 14:21. https://doi.org/10.1186/s12934-015-0205-9 - DOI

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