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. 2024 Feb 15;187(4):846-860.e17.
doi: 10.1016/j.cell.2023.12.033. Epub 2024 Jan 22.

Cell surface RNAs control neutrophil recruitment

Ningning Zhang  1 , Wenwen Tang  2 , Lidiane Torres  3 , Xujun Wang  1 , Yasmeen Ajaj  1 , Li Zhu  4 , Yi Luan  2 , Hongyue Zhou  2 , Yadong Wang  5 , Dingyao Zhang  6 , Vadim Kurbatov  7 , Sajid A Khan  8 , Priti Kumar  4 , Andres Hidalgo  9 , Dianqing Wu  10 , Jun Lu  11
Affiliations

Cell surface RNAs control neutrophil recruitment

Ningning Zhang et al. Cell. .

Abstract

RNAs localizing to the outer cell surface have been recently identified in mammalian cells, including RNAs with glycan modifications known as glycoRNAs. However, the functional significance of cell surface RNAs and their production are poorly known. We report that cell surface RNAs are critical for neutrophil recruitment and that the mammalian homologs of the sid-1 RNA transporter are required for glycoRNA expression. Cell surface RNAs can be readily detected in murine neutrophils, the elimination of which substantially impairs neutrophil recruitment to inflammatory sites in vivo and reduces neutrophils' adhesion to and migration through endothelial cells. Neutrophil glycoRNAs are predominantly on cell surface, important for neutrophil-endothelial interactions, and can be recognized by P-selectin (Selp). Knockdown of the murine Sidt genes abolishes neutrophil glycoRNAs and functionally mimics the loss of cell surface RNAs. Our data demonstrate the biological importance of cell surface glycoRNAs and highlight a noncanonical dimension of RNA-mediated cellular functions.

Keywords: 45S rRNA; E-selectin; Sidt1; Sidt2; peritonitis; snoRNA; tRNA.

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Conflict of interest statement

Declaration of interests The authors declare no competing interests.

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