Preeclampsia impedes foetal kidney development by delivering placenta-derived exosomes to glomerular endothelial cells
- PMID: 37996949
- PMCID: PMC10666440
- DOI: 10.1186/s12964-023-01286-y
Preeclampsia impedes foetal kidney development by delivering placenta-derived exosomes to glomerular endothelial cells
Erratum in
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Correction: Preeclampsia impedes foetal kidney development by delivering placenta-derived exosomes to glomerular endothelial cells.Cell Commun Signal. 2024 Jun 7;22(1):316. doi: 10.1186/s12964-024-01697-5. Cell Commun Signal. 2024. PMID: 38849844 Free PMC article. No abstract available.
Abstract
Background: Foetal renal dysplasia is still the main cause of adult renal disease. Placenta-derived exosomes are an important communication tool, and they may play an important role in placental (both foetal and maternal) function. We hypothesize that in women with preeclampsia, foetal renal dysplasia is impeded by delivering placenta-derived exosomes to glomerular endothelial cells.
Methods: In the present study, we established a PE trophoblast oxidative stress model to isolate exosomes from supernatants by ultracentrifugation (NO-exo and H/R-exo) and collected normal and PE umbilical cord blood plasma to isolate exosomes by ultracentrifugation combined with sucrose density gradient centrifugation (N-exo and PE-exo), then we investigated their effects on foetal kidney development by in vitro, ex vivo and in vivo models.
Results: The PE trophoblast oxidative stress model was established successfully. After that, in in vitro studies, we found that H/R-exo and PE-exo could adversely affect glomerular endothelial cell proliferation, tubular formation, migration, and barrier functions. In ex vivo studies, H/R-exo and PE-exo both inhibited the growth and branch formation of kidney explants, along with the decrease of VE-cadherin and Occludin. In in vivo studies, we also found that H/R-exo and PE-exo could result in renal dysplasia, reduced glomerular number, and reduced barrier function in foetal mice.
Conclusions: In conclusion, we demonstrated that PE placenta-derived exosomes could lead to foetal renal dysplasia by delivering placenta-derived exosomes to foetal glomerular endothelial cells, which provides a novel understanding of the pathogenesis of foetal renal dysplasia. Video Abstract.
Keywords: Foetal renal dysplasia; Human glomerular endothelial cell; Hypoxia; Placenta-derived exosomes; Preeclampsia; Reoxygenation.
© 2023. The Author(s).
Conflict of interest statement
The authors report no conflicts of interest in this work.
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