High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq
- PMID: 36823353
- PMCID: PMC10567548
- DOI: 10.1038/s41587-023-01676-0
High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq
Abstract
In this study, we extended co-indexing of transcriptomes and epitopes (CITE) to the spatial dimension and demonstrated high-plex protein and whole transcriptome co-mapping. We profiled 189 proteins and whole transcriptome in multiple mouse tissue types with spatial CITE sequencing and then further applied the method to measure 273 proteins and transcriptome in human tissues, revealing spatially distinct germinal center reactions in tonsil and early immune activation in skin at the Coronavirus Disease 2019 mRNA vaccine injection site.
© 2023. The Author(s).
Conflict of interest statement
R.F., Y.L. and Y.D. are inventors on a patent application related to this work. R.F. is scientific founder and advisor of IsoPlexis, Singleron Biotechnologies and AtlasXomics. The interests of R.F. were reviewed and managed by the Yale University Provost’s Office in accordance with the university’s conflict of interest policies. P.B. and M.C. are employees of Lunaphore Technologies SA. The remaining authors declare no competing interests.
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Update of
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Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping.Res Sq [Preprint]. 2022 Mar 30:rs.3.rs-1499315. doi: 10.21203/rs.3.rs-1499315/v1. Res Sq. 2022. Update in: Nat Biotechnol. 2023 Oct;41(10):1405-1409. doi: 10.1038/s41587-023-01676-0. PMID: 35378748 Free PMC article. Updated. Preprint.
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