Role of cathepsins B and D in proteolysis of yolk in the catfish Clarias gariepinus
- PMID: 35482165
- DOI: 10.1007/s10695-022-01062-8
Role of cathepsins B and D in proteolysis of yolk in the catfish Clarias gariepinus
Abstract
Yolk processing pathways vary in the oocytes of benthophil and pelagophil teleosts. The present study investigated the yolk processing pattern in the oocytes of the fresh water catfish Clarias gariepinus at vitellogenic, maturation, and ovulated stages. This study concludes that during maturation stage, an electrophoretic shift in the major peptide band on Polyacrylamide gel electrophoresis (PAGE) occurs due to a decrease in the size of the yolk protein. The PMF spectrum of corresponding peptides from vitellogenic and ovulated oocytes revealed a difference in the minor ions. A minor difference in the molecular weight of the corresponding peptides occurs due to a difference in their amino acid composition. Maximal activity of the proteases cathepsin D and cathepsin B was observed in the vitellogenic oocytes, thus confirming their role in the processing of yolk. A significant transient increase in the activity of cathepsin B in the mature oocytes also suggests its role in oocyte maturation.
Keywords: Cathepsin B; Cathepsin D; Clarias gariepinus; Maturation; Oocytes; Ovulation; Yolk protein.
© 2022. The Author(s), under exclusive licence to Springer Nature B.V.
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