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Review
. 2021 Nov;82(11):801-811.
doi: 10.1016/j.humimm.2021.02.012. Epub 2021 Mar 19.

Next-generation sequencing technologies: An overview

Taishan Hu  1 , Nilesh Chitnis  2 , Dimitri Monos  3 , Anh Dinh  4
Affiliations
Review

Next-generation sequencing technologies: An overview

Taishan Hu et al. Hum Immunol. 2021 Nov.

Abstract

Since the days of Sanger sequencing, next-generation sequencing technologies have significantly evolved to provide increased data output, efficiencies, and applications. These next generations of technologies can be categorized based on read length. This review provides an overview of these technologies as two paradigms: short-read, or "second-generation," technologies, and long-read, or "third-generation," technologies. Herein, short-read sequencing approaches are represented by the most prevalent technologies, Illumina and Ion Torrent, and long-read sequencing approaches are represented by Pacific Biosciences and Oxford Nanopore technologies. All technologies are reviewed along with reported advantages and disadvantages. Until recently, short-read sequencing was thought to provide high accuracy limited by read-length, while long-read technologies afforded much longer read-lengths at the expense of accuracy. Emerging developments for third-generation technologies hold promise for the next wave of sequencing evolution, with the co-existence of longer read lengths and high accuracy.

Keywords: Long-read sequencing; Next-generation sequencing; Short-read sequencing.

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Conflict of interest statement

Declaration of Competing Interest Dimitri Monos receives royalties from, is a consultant to, and owns options in Omixon Inc. The remaining authors have no conflicts of interest to disclose.

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