Expression analysis of mammalian mitochondrial ribosomal protein genes
- PMID: 32987154
- PMCID: PMC7726062
- DOI: 10.1016/j.gep.2020.119147
Expression analysis of mammalian mitochondrial ribosomal protein genes
Abstract
Mitochondrial ribosomal proteins (MRPs) are essential components for the structural and functional integrity of the mitoribosome complex. Throughout evolution, the mammalian mitoribosome has acquired new Mrp genes to compensate for loss of ribosomal RNA. More than 80 MRPs have been identified in mammals. Here we document expression pattern of 79 Mrp genes during mouse development and adult tissues and find that these genes are consistently expressed throughout early embryogenesis with little stage or tissue specificity. Further investigation of the amino acid sequence reveals that this group of proteins has little to no protein similarity. Recent work has shown that the majority of Mrp genes are essential resulting in early embryonic lethality, suggesting no functional redundancy among the group. Taken together, these results indicate that the Mrp genes are not a gene family descended from a single ancestral gene, and that each MRP has unique and essential role in the mitoribosome complex. The lack of functional redundancy is surprising given the importance of the mitoribosome for cellular and organismal viability. Further, these data suggest that genomic variants in Mrp genes may be causative for early pregnancy loss and should be evaluated as clinically.
Keywords: MRP; Mitochondria; Mitoribosome complex; Mrpl1; Mrpl10; Mrpl11; Mrpl12; Mrpl13; Mrpl14; Mrpl15; Mrpl16; Mrpl17; Mrpl18; Mrpl19; Mrpl2; Mrpl20; Mrpl21; Mrpl22; Mrpl23; Mrpl24; Mrpl27; Mrpl28; Mrpl3; Mrpl30; Mrpl32; Mrpl33; Mrpl34; Mrpl35; Mrpl36; Mrpl37; Mrpl38; Mrpl39; Mrpl4; Mrpl40; Mrpl41; Mrpl42; Mrpl43; Mrpl44; Mrpl45; Mrpl46; Mrpl47; Mrpl48; Mrpl49; Mrpl50; Mrpl51; Mrpl52; Mrpl53; Mrpl54; Mrpl55; Mrpl56; Mrpl57; Mrpl58; Mrpl59; Mrpl9; Mrps10; Mrps11; Mrps12; Mrps14; Mrps15; Mrps16; Mrps17; Mrps18a; Mrps18b; Mrps18c; Mrps2; Mrps21; Mrps22; Mrps23; Mrps24; Mrps25; Mrps26; Mrps27; Mrps28; Mrps29; Mrps30; Mrps31; Mrps33; Mrps34; Mrps35; Mrps36; Mrps37; Mrps38; Mrps39; Mrps5; Mrps6; Mrps7; Mrps9.
Copyright © 2020 Elsevier B.V. All rights reserved.
Conflict of interest statement
Conflict statement: We have no conflict of interest or the appearance of conflict of interest with the data presented in this manuscript.
Figures
![Figure 1.](https://cdn.statically.io/img/www.ncbi.nlm.nih.gov/pmc/articles/instance/7726062/bin/nihms-1634075-f0001.gif)
![Figure 2.](https://cdn.statically.io/img/www.ncbi.nlm.nih.gov/pmc/articles/instance/7726062/bin/nihms-1634075-f0002.gif)
Similar articles
-
The role of the mitochondrial ribosomal protein family in detecting hepatocellular carcinoma and predicting prognosis, immune features, and drug sensitivity.Clin Transl Oncol. 2024 Feb;26(2):496-514. doi: 10.1007/s12094-023-03269-4. Epub 2023 Jul 5. Clin Transl Oncol. 2024. PMID: 37407805
-
Nuclear-encoded mitochondrial ribosomal proteins are required to initiate gastrulation.Development. 2020 May 26;147(10):dev188714. doi: 10.1242/dev.188714. Development. 2020. PMID: 32376682 Free PMC article.
-
Expression and affinity purification of recombinant mammalian mitochondrial ribosomal small subunit (MRPS) proteins and protein-protein interaction analysis indicate putative role in tumourigenic cellular processes.J Biochem. 2021 Sep 7;169(6):675-692. doi: 10.1093/jb/mvab004. J Biochem. 2021. PMID: 34492114
-
Mammalian mitochondrial ribosomal small subunit (MRPS) genes: A putative role in human disease.Gene. 2016 Sep 1;589(1):27-35. doi: 10.1016/j.gene.2016.05.008. Epub 2016 May 8. Gene. 2016. PMID: 27170550 Review.
-
Nuclear MRP genes and mitochondrial disease.Gene. 2005 Jul 18;354:147-51. doi: 10.1016/j.gene.2005.03.026. Gene. 2005. PMID: 15908146 Review.
Cited by
-
Illuminating mitochondrial translation through mouse models.Hum Mol Genet. 2024 May 22;33(R1):R61-R79. doi: 10.1093/hmg/ddae020. Hum Mol Genet. 2024. PMID: 38779771 Free PMC article. Review.
-
MRPS16 promotes lung adenocarcinoma growth via the PI3K/AKT/Frataxin signalling axis.J Cell Mol Med. 2024 Apr;28(7):e18166. doi: 10.1111/jcmm.18166. J Cell Mol Med. 2024. PMID: 38506080 Free PMC article.
-
MRPS9-Mediated Regulation of the PI3K/Akt/mTOR Pathway Inhibits Neuron Apoptosis and Protects Ischemic Stroke.J Mol Neurosci. 2024 Feb 21;74(1):23. doi: 10.1007/s12031-024-02197-4. J Mol Neurosci. 2024. PMID: 38381220
-
Human retinal organoids with an OPA1 mutation are defective in retinal ganglion cell differentiation and function.Stem Cell Reports. 2024 Jan 9;19(1):68-83. doi: 10.1016/j.stemcr.2023.11.004. Epub 2023 Dec 14. Stem Cell Reports. 2024. PMID: 38101398 Free PMC article.
-
Integration of transcriptomic, proteomic, and metabolomic data to identify lncRNA rPvt1 associations in lipopolysaccharide-treated H9C2 cardiomyocytes.Front Genet. 2023 Nov 29;14:1278830. doi: 10.3389/fgene.2023.1278830. eCollection 2023. Front Genet. 2023. PMID: 38094756 Free PMC article.
References
-
- Lang MWGBF, and Burger G, Mitochondrial Genome Evolution and the Origin of Eukaryotes. Annual review of genetics 33, 351–397 (1999). - PubMed
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases