Molecular composition of luteinizing hormone and follicle-stimulating hormone in commercial gonadotropin preparations
- PMID: 3096793
- DOI: 10.1016/s0015-0282(16)49879-1
Molecular composition of luteinizing hormone and follicle-stimulating hormone in commercial gonadotropin preparations
Abstract
The biologic (B) and immunologic (I) properties of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were studied in three commercial urinary gonadotropin preparations and in the first international standard preparation of human urinary gonadotropins before and after fractionation by isoelectrofocusing (IEF). Significant differences were found in the IEF profiles of both bioactive and immunoreactive LH and FSH and in the B/I ratios of the preparations studied. The observed differences in the molecular composition of LH and FSH seem to be attributable to the purification procedures employed. The possible influence of these differences on the in vivo potencies, circulating half-lives, and clinical effects of gonadotropin preparations are discussed.
Similar articles
-
Follicular fluid hormone concentrations after ovarian stimulation using gonadotropin preparations with different FSH/LH ratios. II. Comparison of hMG and recombinant FSH.Int J Fertil Womens Med. 1997 Nov-Dec;42(6):431-5. Int J Fertil Womens Med. 1997. PMID: 9459089 Clinical Trial.
-
Biological and immunological characterization of purified follicle-stimulating hormone preparations from monkey pituitaries.J Med Primatol. 1984;13(5):269-82. J Med Primatol. 1984. PMID: 6439872
-
Influence of gonadectomy on isoelectrofocusing profiles of pituitary gonadotropins in rhesus monkeys.J Med Primatol. 1985;14(4):177-94. J Med Primatol. 1985. PMID: 3930752
-
Immunological and biological potencies of the different molecular species of gonadotrophins.Hum Reprod. 1988 May;3(4):491-501. doi: 10.1093/oxfordjournals.humrep.a136734. Hum Reprod. 1988. PMID: 3134393 Review.
-
Human gonadotropins: biochemical, immunological, and clinical aspects.Adv Sex Horm Res. 1976;2:57-85. Adv Sex Horm Res. 1976. PMID: 797249 Review.
Cited by
-
Hormonal Stimulation of Human Ovarian Xenografts in Mice: Studying Folliculogenesis, Activation, and Oocyte Maturation.Endocrinology. 2020 Dec 1;161(12):bqaa194. doi: 10.1210/endocr/bqaa194. Endocrinology. 2020. PMID: 33099627 Free PMC article. Review.
-
Biological activity and metabolic clearance of recombinant human follicle stimulating hormone produced in Sp2/0 myeloma cells.Cytotechnology. 1996 Jun;21(2):171-82. doi: 10.1007/BF02215667. Cytotechnology. 1996. PMID: 22358666
-
Establishment of an in vitro bioassay and radio receptor assay for LH/CG in human sera using immortalized granulosa cells transfected with LH/CG receptor.Endocrine. 1996 Dec;5(3):275-83. doi: 10.1007/BF02739060. Endocrine. 1996. PMID: 21153078
-
Molecular composition of two different batches of urofollitropin: analysis by immunofluorimetric assay, radioligand receptor assay and in vitro bioassay.J Endocrinol Invest. 1993 Jan;16(1):21-7. doi: 10.1007/BF03345823. J Endocrinol Invest. 1993. PMID: 8445153
-
Glycoprotein hormone isomorphism and assay discrepancy: the paradigm of luteinizing hormone (LH).J Endocrinol Invest. 1994 Apr;17(4):291-9. doi: 10.1007/BF03348981. J Endocrinol Invest. 1994. PMID: 7523478 Review. No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
