Next-Generation Sequencing Strategies
- PMID: 30323017
- PMCID: PMC6601457
- DOI: 10.1101/cshperspect.a025791
Next-Generation Sequencing Strategies
Abstract
More than a decade ago, the term "next-generation" sequencing was coined to describe what was, at the time, revolutionary new methods to sequence RNA and DNA at a faster pace and cheaper cost than could be performed by standard bench-top protocols. Since then, the field of DNA sequencing has evolved at a rapid pace, with new breakthroughs allowing capacity to exponentially increase and cost to dramatically decrease. As genome-scale sequencing has become routine, a paradigm shift is occurring in genomics, which uses the power of high-throughput, rapid sequencing power with large-scale studies. These new approaches to genetic discovery will provide direct impact to fields such as personalized medicine, evolution, and biodiversity. This work reviews recent technology advances and methods in next-generation sequencing and highlights current large-scale sequencing efforts driving the evolution of the genomics space.
Copyright © 2019 Cold Spring Harbor Laboratory Press; all rights reserved.
Similar articles
-
Advancements in Next-Generation Sequencing.Annu Rev Genomics Hum Genet. 2016 Aug 31;17:95-115. doi: 10.1146/annurev-genom-083115-022413. Epub 2016 Jun 9. Annu Rev Genomics Hum Genet. 2016. PMID: 27362342 Review.
-
Future Promises and Concerns of Ubiquitous Next-Generation Sequencing.Cold Spring Harb Perspect Med. 2019 Sep 3;9(9):a025783. doi: 10.1101/cshperspect.a025783. Cold Spring Harb Perspect Med. 2019. PMID: 30478095 Free PMC article. Review.
-
Ten years of next-generation sequencing technology.Trends Genet. 2014 Sep;30(9):418-26. doi: 10.1016/j.tig.2014.07.001. Epub 2014 Aug 6. Trends Genet. 2014. PMID: 25108476 Review.
-
Next-generation sequencing and potential applications in fungal genomics.Methods Mol Biol. 2011;722:51-60. doi: 10.1007/978-1-61779-040-9_4. Methods Mol Biol. 2011. PMID: 21590412
-
Next-Generation Sequencing and Emerging Technologies.Semin Thromb Hemost. 2019 Oct;45(7):661-673. doi: 10.1055/s-0039-1688446. Epub 2019 May 16. Semin Thromb Hemost. 2019. PMID: 31096307 Review.
Cited by
-
Development and emerging trends of drug resistance mutations in HIV: a bibliometric analysis based on CiteSpace.Front Microbiol. 2024 May 15;15:1374582. doi: 10.3389/fmicb.2024.1374582. eCollection 2024. Front Microbiol. 2024. PMID: 38812690 Free PMC article.
-
Technical Advances in Circulating Cell-Free DNA Detection and Analysis for Personalized Medicine in Patients' Care.Biomolecules. 2024 Apr 19;14(4):498. doi: 10.3390/biom14040498. Biomolecules. 2024. PMID: 38672514 Free PMC article. Review.
-
SARS-CoV-2 resistance to monoclonal antibodies and small-molecule drugs.Cell Chem Biol. 2024 Apr 18;31(4):632-657. doi: 10.1016/j.chembiol.2024.03.008. Cell Chem Biol. 2024. PMID: 38640902 Review.
-
Advances in Brain-Gut-Microbiome Interactions: A Comprehensive Update on Signaling Mechanisms, Disorders, and Therapeutic Implications.Cell Mol Gastroenterol Hepatol. 2024;18(1):1-13. doi: 10.1016/j.jcmgh.2024.01.024. Epub 2024 Feb 8. Cell Mol Gastroenterol Hepatol. 2024. PMID: 38336171 Free PMC article. Review.
-
Two decades of population genomics: will we ever agree on bacterial species?BMC Biol. 2024 Jan 26;22(1):20. doi: 10.1186/s12915-023-01797-7. BMC Biol. 2024. PMID: 38279118 Free PMC article.
References
-
- Afshinnekoo E, Ahsanuddin S, Mason CE. 2016. Globalizing and crowdsourcing biomedical research. Br Med Bull 120: 27–33. - PubMed
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Research Materials