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. 2017 Mar;137(3):751-753.
doi: 10.1016/j.jid.2016.10.037. Epub 2016 Nov 8.

FISH Panel for Leukemic CTCL

Jason Weed  1 , Juliet Gibson  1 , Julia Lewis  1 , Kacie Carlson  1 , Francine Foss  1 , Jaehyuk Choi  2 , Peining Li  3 , Michael Girardi  4
Affiliations

FISH Panel for Leukemic CTCL

Jason Weed et al. J Invest Dermatol. 2017 Mar.
No abstract available

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Conflict of interest statement

CONFLICTS OF INTEREST:

JW, JL, and MG are listed as inventors on a provisional patent filed on several probes reported herein.

Figures

Figure 1
Figure 1. L-CTCL FISH panel composition and probe microscopy
(a) Table of genes, chromosomal locations, and fluorescent colors for 11 clinically validated probes used in the CTCL FISH panel. (b–g) Post-hybridization microscopy images using 11-probe FISH panel on peripheral blood samples from patients with L-CTCL and abnormal FISH results. (b) Field with cell showing normal copy number of two TP53 (red) signals and two ATM (green) signals, and two cells with deletion of one copy of TP53. (c) Cells with two copies of CDKN2A (red) and 9q12 control probe (green). (d) Two cells with two copies of RB1 (red) and 13q control (green). (e) Signals for DMNT3A (green), CARD11 (aqua), and FAS (red), both cells showing two copies of each. (f) Cell on top with two copies of MYC stained with the red and green probes (both for MYC); cell below showing four copies of MYC. (g) Signals for ARID1A (red), ZEB1 (green), and STAT3/5B (aqua); one cell with normal copy numbers and two cells with homozygous deletion of ZEB1 and amplification of STAT3/5B. Black bar = 10 μm.
Figure 2
Figure 2. Distribution of deletions and amplifications detected by FISH
(a) Heatmap of deletions and amplification in CTCL driver genes detected by the 11-probe CTCL FISH panel among 10 patients with SS, 1 patient with F-MF, 1 patient with MF-T, and 2 patients with MF. Each column shows FISH status for one patient. (b) Comparison of proportion (+/− standard error) of SS patients with abnormal copy numbers in each gene by FISH (n=10) versus CTCL exome sequencing (n=40) on non-overlapping patient cohorts. No significant differences were found for any gene (Fisher’s exact test, p > 0.1). *For STAT3/5B FISH probe, comparator gene from exome data is STAT5B.
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