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. 2015 Feb 2:6:20.
doi: 10.3389/fmicb.2015.00020. eCollection 2015.

Microbiota disbiosis is associated with colorectal cancer

Zhiguang Gao  1 , Bomin Guo  1 , Renyuan Gao  1 , Qingchao Zhu  1 , Huanlong Qin  1
Affiliations

Microbiota disbiosis is associated with colorectal cancer

Zhiguang Gao et al. Front Microbiol. .

Abstract

The dysbiosis of the human intestinal microbiota is linked to sporadic colorectal carcinoma (CRC). The present study was designed to investigate the gut microbiota distribution features in CRC patients. We performed pyrosequencing based analysis of the 16S rRNA gene V3 region to investigate microbiota of the cancerous tissue and adjacent non-cancerous normal tissue in proximal and distal CRC samples. The results revealed that the microbial structures of the CRC patients and healthy individuals differed significantly. Firmicutes and Fusobacteria were over-represented whereas Proteobacteria was under-represented in CRC patients. In addition, Lactococcus and Fusobacterium exhibited a relatively higher abundance while Pseudomonas and Escherichia-Shigella was reduced in cancerous tissues compared to adjacent non-cancerous tissues. Meanwhile, the overall microbial structures of proximal and distal colon cancerous tissues were similar; but certain potential pro-oncogenic pathogens were different. These results suggested that the mucosa-associated microbiota is dynamically associated with CRC, which may provide evidences for microbiota-associated diagnostic, prognostic, preventive, and therapeutic strategies for CRC.

Keywords: colorectal cancer; distal colon; gut dysbiosis; mucosa-associated microbiota; proximal colon.

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Figures

Figure 1
Figure 1
Alpha-diversity distances calculated using phylotype relative abundance measurements between healthy and CRC groups demonstrate that the microbial richness of CRC patients is higher than healthy individuals, while the diversity has no statistical significance between two groups.
Figure 2
Figure 2
Different structures of gut microbiota between healthy individuals and CRC patients. (A) The dominant phyla of group tumor and healthy. (B) The dominant genera of group tumor and healthy. (C) The dominant phyla of group cancer and non-cancerous mucosa. (D) The dominant genera of group cancer and cancerous mucosa. (E) Hierarchical clustering of phylotype relative abundance measurements demonstrates that microbial composition of tumor samples from different individuals is more highly correlated than tumor/health samples within individuals. (F) Hierarchical clustering of proximal and distal CRC.
Figure 3
Figure 3
Histogram of the LDA scores for differentially abundant genera. Cladogram was calculated by LEfSe, a metagenome analysis approach.
Figure 4
Figure 4
Principal component analysis (PCA) scores plot based on the relative abundance of OTUs (97% similarity level). Each symbol represents a sample. Green circles represent proximal CRC. Red quadrates represent distal CRC.
See this image and copyright information in PMC

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References

    1. Allen V. E., Strauss J., Chadee K. (2011). Fusobacterium nucleatum: an emerging gut pathogen? Gut Microbes 2, 294–298. 10.4161/gmic.2.5.18603 - DOI - PubMed
    1. Bachrach G., Ianculovici C., Naor R., Weiss E. I. (2005). Fluorescence-based measurements of Fusobacterium nucleatum coaggregation and of fusobacterial attachment to mammalian cells. FEMS Microbiol. Lett. 248, 235–240. 10.1016/j.femsle.2005.05.055 - DOI - PubMed
    1. Borugian M. J., Sheps S. B., Whittemore A. S., Wu A. H., Potter J. D., Gallagher R. P. (2002). Carbohydrates and colorectal cancer risk among Chinese in North America Cancer. Epidemiol. Biomarkers Prev. 11, 187–193. - PubMed
    1. Brenner H., Kloor M., Pox C. P. (2014). Colorectal cancer. Lancet 383, 1490–1502. 10.1016/S0140-6736(13)61649-9 - DOI - PubMed
    1. Bufill J. A. (1990). Colorectal cancer: evidence for distinct genetic categories based on proximal or distal tumor location. Ann. Intern. Med. 113, 779–788. 10.7326/0003-4819-113-10-779 - DOI - PubMed