Mouse models of nonmelanoma skin cancer
- PMID: 25636471
- DOI: 10.1007/978-1-4939-2297-0_10
Mouse models of nonmelanoma skin cancer
Abstract
The skin is the largest organ of the mammalian body, made up of multiple layers, which include the epidermis, dermis, and subcutis (Alam and Ratner, N Engl J Med 344(13):975-983, 2001). The human interfollicular epidermis can be subdivided into five different layers: (1) stratum basale, (2) stratum spinosum, (3) stratum granulosum, (4) stratum lucidum, and (5) stratum corneum, all originating from basal keratinocytes by differentiation (Hameetman et al., BMC cancer 13:58, 2013; Ramirez et al., Differentiation 58(1):53-64, 1994). The epidermis is also able to generate different appendages: hair follicles (HF) and their associated sebaceous glands (Sibilia et al., Cell 102(2):211-220, 2000) as well as sweat glands (Luetteke et al., Genes Dev 8(4):399-413, 1994). The skin has important functions in several biological processes like environmental barrier, tissue regeneration, hair cycling, and wound repair. During these processes, stem cells from the interfollicular epidermis and from the hair follicle bulge are activated to renew the epidermis or hair. The epidermis and hair undergo continuous homeostatic regeneration and mutations, upon mutations which disturb the balance of homeostatic regeneration of epidermis and hair and lead to enhanced proliferation of keratinocytes, development of skin cancer is developed. Tumors that arise in the skin are mainly of three types: malignant melanoma, arising from melanocytes, basal cell carcinoma (BCC), and squamous cell carcinoma (SCC), the latter two both arising from keratinocytes or hair follicle cells. In this chapter, we will describe some genetically engineered mouse models (GEMM) that aim at modeling human BCC and SCC and their respective precancerous lesions. We will describe the experimental approaches used in our laboratory to analyze tumor-bearing mice focusing on methods necessary for the induction of tumor growth as well as for the molecular and histological analysis of tumor tissue.
Similar articles
-
Evidence that the epidermal targets of carcinogen action are found in the interfollicular epidermis of infundibulum as well as in the hair follicles.Cancer Res. 2000 Jan 15;60(2):226-9. Cancer Res. 2000. PMID: 10667563
-
Early epidermal destruction with subsequent epidermal hyperplasia is a unique feature of the papilloma-independent squamous cell carcinoma phenotype in PKCepsilon overexpressing transgenic mice.Toxicol Pathol. 2005;33(6):684-94. doi: 10.1080/01926230500323441. Toxicol Pathol. 2005. PMID: 16243773
-
Crosstalk between Desmoglein 2 and Patched 1 accelerates chemical-induced skin tumorigenesis.Oncotarget. 2015 Apr 20;6(11):8593-605. doi: 10.18632/oncotarget.3309. Oncotarget. 2015. PMID: 25871385 Free PMC article.
-
Beauty is skin deep: the fascinating biology of the epidermis and its appendages.Harvey Lect. 1998-1999;94:47-77. Harvey Lect. 1998. PMID: 11070952 Review.
-
Non-melanoma skin cancer in mouse and man.Arch Toxicol. 2013 May;87(5):783-98. doi: 10.1007/s00204-012-0998-9. Epub 2012 Dec 25. Arch Toxicol. 2013. PMID: 23266722 Review.
Cited by
-
Oncogenic Kras induces spatiotemporally specific tissue deformation through converting pulsatile into sustained ERK activation.Nat Cell Biol. 2024 Jun;26(6):859-867. doi: 10.1038/s41556-024-01413-y. Epub 2024 Apr 30. Nat Cell Biol. 2024. PMID: 38689013
-
Multi-Tissue Characterization of GILZ Expression in Dendritic Cell Subsets at Steady State and in Inflammatory Contexts.Cells. 2021 Nov 13;10(11):3153. doi: 10.3390/cells10113153. Cells. 2021. PMID: 34831376 Free PMC article.
-
A genome-wide library of MADM mice for single-cell genetic mosaic analysis.Cell Rep. 2021 Jun 22;35(12):109274. doi: 10.1016/j.celrep.2021.109274. Cell Rep. 2021. PMID: 34161767 Free PMC article.
-
Basal Cell Carcinoma Arises from Interfollicular Layer of Epidermis.J Oncol. 2018 Sep 26;2018:3098940. doi: 10.1155/2018/3098940. eCollection 2018. J Oncol. 2018. PMID: 30356421 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Research Materials
Miscellaneous