In vitro colonic catabolism of orange juice (poly)phenols
- PMID: 25545994
- DOI: 10.1002/mnfr.201400779
In vitro colonic catabolism of orange juice (poly)phenols
Abstract
Scope: The role of colonic microbiota in the breakdown of hesperetin, naringenin, and ferulic acid, compounds found as glycosides in orange juice, was investigated using an in vitro fermentation model.
Methods and results: Test compounds were incubated with human fecal slurries cultured under anaerobic conditions, and the production of phenolic acid catabolites were monitored by GC-MS and HPLC-MS(2) . Hesperetin was converted to 3-(3'-hydroxy-4'-methoxyphenyl)propionic acid, 3-(3',4'-dihydroxyphenyl)propionic acid, and 3-(3'-hydroxyphenyl)propionic acid while 3-(phenyl)propionic acid was the major end product derived from naringenin. The data obtained are compared to our previously published data on urinary excretion of phenolic and aromatic acids after acute orange juice consumption (Pereira-Caro et al. Am. J. Clin. Nutr. 2014, 100, 1385-1391). Catabolism pathways are proposed for events occurring in the colon and those taking place postabsorption into the circulatory system with particular reference to the excretion of 3-(3'-hydroxy-4'-methoxyphenyl)hydracrylic acid, which is not formed in fecal incubations. Ferulic acid was also degraded by the colonic microflora being converted principally to 3-(3'-methoxy-4'-hydroxyphenyl)propionic acid, a phenolic acid that appears in urine after orange juice consumption.
Conclusion: The study provides novel information on the potential involvement of the colonic microbiota in the overall bioavailability of orange juice (poly)phenols through the production of phenylpropionic acids and subsequent hepatic conversions that lead to hippuric acid and its hydroxylated analogues.
Keywords: 3-(Phenyl)hydracrylic acids; 3-(Phenyl)propionic acids; Colonic catabolites; Ferulic acid; Flavanones; Human gut microflora; In vitro fecal fermentation.
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Similar articles
-
Orange juice (poly)phenols are highly bioavailable in humans.Am J Clin Nutr. 2014 Nov;100(5):1378-84. doi: 10.3945/ajcn.114.090282. Epub 2014 Aug 27. Am J Clin Nutr. 2014. PMID: 25332336
-
Yoghurt impacts on the excretion of phenolic acids derived from colonic breakdown of orange juice flavanones in humans.Mol Nutr Food Res. 2009 May;53 Suppl 1:S68-75. doi: 10.1002/mnfr.200800287. Mol Nutr Food Res. 2009. PMID: 19415668
-
Bioavailability of orange juice (poly)phenols: the impact of short-term cessation of training by male endurance athletes.Am J Clin Nutr. 2017 Sep;106(3):791-800. doi: 10.3945/ajcn.116.149898. Epub 2017 Jul 26. Am J Clin Nutr. 2017. PMID: 28747329 Clinical Trial.
-
Colonic metabolites of berry polyphenols: the missing link to biological activity?Br J Nutr. 2010 Oct;104 Suppl 3:S48-66. doi: 10.1017/S0007114510003946. Br J Nutr. 2010. PMID: 20955650 Review.
-
Role of the small intestine, colon and microbiota in determining the metabolic fate of polyphenols.Biochem Pharmacol. 2017 Sep 1;139:24-39. doi: 10.1016/j.bcp.2017.03.012. Epub 2017 Mar 18. Biochem Pharmacol. 2017. PMID: 28322745 Review.
Cited by
-
Flavouring Group Evaluation 413 (FGE.413): Naringenin.EFSA J. 2024 May 15;22(5):e8747. doi: 10.2903/j.efsa.2024.8747. eCollection 2024 May. EFSA J. 2024. PMID: 38751504 Free PMC article.
-
Neuroprotective Potentials of Flavonoids: Experimental Studies and Mechanisms of Action.Antioxidants (Basel). 2023 Jan 27;12(2):280. doi: 10.3390/antiox12020280. Antioxidants (Basel). 2023. PMID: 36829840 Free PMC article. Review.
-
Fermentation Extract of Naringenin Increases the Expression of Estrogenic Receptor β and Modulates Genes Related to the p53 Signalling Pathway, miR-200c and miR-141 in Human Colon Cancer Cells Exposed to BPA.Molecules. 2022 Oct 5;27(19):6588. doi: 10.3390/molecules27196588. Molecules. 2022. PMID: 36235125 Free PMC article.
-
Contribution of the diverse experimental models to unravelling the biological scope of dietary (poly)phenols.J Sci Food Agric. 2022 Aug 15;102(10):3940-3951. doi: 10.1002/jsfa.11865. Epub 2022 Mar 31. J Sci Food Agric. 2022. PMID: 35285937 Free PMC article. Review.
-
Beneficial effects of citrus flavanones naringin and naringenin and their food sources on lipid metabolism: An update on bioavailability, pharmacokinetics, and mechanisms.J Nutr Biochem. 2022 Jun;104:108967. doi: 10.1016/j.jnutbio.2022.108967. Epub 2022 Feb 18. J Nutr Biochem. 2022. PMID: 35189328 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Miscellaneous