Potential of fecal microbiota for early-stage detection of colorectal cancer
- PMID: 25432777
- PMCID: PMC4299606
- DOI: 10.15252/msb.20145645
Potential of fecal microbiota for early-stage detection of colorectal cancer
Abstract
Several bacterial species have been implicated in the development of colorectal carcinoma (CRC), but CRC-associated changes of fecal microbiota and their potential for cancer screening remain to be explored. Here, we used metagenomic sequencing of fecal samples to identify taxonomic markers that distinguished CRC patients from tumor-free controls in a study population of 156 participants. Accuracy of metagenomic CRC detection was similar to the standard fecal occult blood test (FOBT) and when both approaches were combined, sensitivity improved > 45% relative to the FOBT, while maintaining its specificity. Accuracy of metagenomic CRC detection did not differ significantly between early- and late-stage cancer and could be validated in independent patient and control populations (N = 335) from different countries. CRC-associated changes in the fecal microbiome at least partially reflected microbial community composition at the tumor itself, indicating that observed gene pool differences may reveal tumor-related host-microbe interactions. Indeed, we deduced a metabolic shift from fiber degradation in controls to utilization of host carbohydrates and amino acids in CRC patients, accompanied by an increase of lipopolysaccharide metabolism.
Keywords: cancer screening; colorectal cancer; fecal biomarkers; human gut microbiome; metagenomics.
© 2014 The Authors. Published under the terms of the CC BY 4.0 license.
Figures
Similar articles
-
Applying simple linear combination, multiple logistic and factor analysis methods for candidate fecal bacteria as novel biomarkers for early detection of adenomatous polyps and colon cancer.J Microbiol Methods. 2018 Dec;155:82-88. doi: 10.1016/j.mimet.2018.11.007. Epub 2018 Nov 12. J Microbiol Methods. 2018. PMID: 30439465
-
Using fecal immunochemical tubes for the analysis of the gut microbiome has the potential to improve colorectal cancer screening.Sci Rep. 2021 Oct 1;11(1):19603. doi: 10.1038/s41598-021-99046-w. Sci Rep. 2021. PMID: 34599256 Free PMC article.
-
Alterations of circulating bacterial DNA in colorectal cancer and adenoma: A proof-of-concept study.Cancer Lett. 2021 Feb 28;499:201-208. doi: 10.1016/j.canlet.2020.11.030. Epub 2020 Nov 27. Cancer Lett. 2021. PMID: 33249197
-
Stool Investigations for Colorectal Cancer Screening: From Occult Blood Test to DNA Analysis.J Gastrointest Cancer. 2016 Jun;47(2):143-51. doi: 10.1007/s12029-016-9810-z. J Gastrointest Cancer. 2016. PMID: 26922358 Review.
-
Fecal occult blood test in colorectal cancer screening.J Dig Dis. 2019 Feb;20(2):62-64. doi: 10.1111/1751-2980.12712. Epub 2019 Mar 5. J Dig Dis. 2019. PMID: 30714325 Review.
Cited by
-
Colorectal cancer microbiome programs DNA methylation of host cells by affecting methyl donor metabolism.Genome Med. 2024 Jun 5;16(1):77. doi: 10.1186/s13073-024-01344-1. Genome Med. 2024. PMID: 38840170 Free PMC article.
-
A review of machine learning methods for cancer characterization from microbiome data.NPJ Precis Oncol. 2024 May 30;8(1):123. doi: 10.1038/s41698-024-00617-7. NPJ Precis Oncol. 2024. PMID: 38816569 Free PMC article. Review.
-
Impact of low-dose ozone nanobubble treatments on antimicrobial resistance genes in pond water.Front Microbiol. 2024 May 13;15:1393266. doi: 10.3389/fmicb.2024.1393266. eCollection 2024. Front Microbiol. 2024. PMID: 38812692 Free PMC article.
-
Exploring Gut Microbiome Composition and Circulating Microbial DNA Fragments in Patients with Stage II/III Colorectal Cancer: A Comprehensive Analysis.Cancers (Basel). 2024 May 18;16(10):1923. doi: 10.3390/cancers16101923. Cancers (Basel). 2024. PMID: 38792001 Free PMC article.
-
Multi-class boosting for the analysis of multiple incomplete views on microbiome data.BMC Bioinformatics. 2024 May 14;25(1):188. doi: 10.1186/s12859-024-05767-w. BMC Bioinformatics. 2024. PMID: 38745112 Free PMC article.
References
-
- Abubucker S, Segata N, Goll J, Schubert AM, Izard J, Cantarel BL, Rodriguez-Mueller B, Zucker J, Thiagarajan M, Henrissat B, White O, Kelley ST, Methe B, Schloss PD, Gevers D, Mitreva M, Huttenhower C. Metabolic reconstruction for metagenomic data and its application to the human microbiome. PLoS Comp Biol. 2012;8:e1002358. - PMC - PubMed
-
- Allison JE, Tekawa IS, Ransom LJ, Adrain AL. A comparison of fecal occult-blood tests for colorectal-cancer screening. New Engl J Med. 1996;334:155–159. - PubMed
-
- Allison JE, Sakoda LC, Levin TR, Tucker JP, Tekawa IS, Cuff T, Pauly MP, Shlager L, Palitz AM, Zhao WK, Schwartz JS, Ransohoff DF, Selby JV. Screening for colorectal neoplasms with new fecal occult blood tests: update on performance characteristics. J Natl Cancer Inst. 2007;99:1462–1470. - PubMed
-
- Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990;215:403–410. - PubMed
-
- Arthur JC, Perez-Chanona E, Muhlbauer M, Tomkovich S, Uronis JM, Fan TJ, Campbell BJ, Abujamel T, Dogan B, Rogers AB, Rhodes JM, Stintzi A, Simpson KW, Hansen JJ, Keku TO, Fodor AA, Jobin C. Intestinal inflammation targets cancer-inducing activity of the microbiota. Science. 2012;338:120–123. - PMC - PubMed
Publication types
MeSH terms
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical