Syncytiotrophoblast-derived microparticle shedding in early-onset and late-onset severe pre-eclampsia
- PMID: 22986096
- DOI: 10.1016/j.ijgo.2012.07.010
Syncytiotrophoblast-derived microparticle shedding in early-onset and late-onset severe pre-eclampsia
Abstract
Objective: To determine the concentration of syncytiotrophoblast-derived microparticles (STBMs) in the maternal circulation and the rate of syncytiotrophoblast apoptosis in the placenta of patients with early-onset and late-onset severe pre-eclampsia.
Methods: A prospective case-control study was conducted that enrolled 15 women with early-onset severe pre-eclampsia, 15 women with late-onset severe pre-eclampsia, and 10 women with normal pregnancies. Plasma STBM levels were measured by enzyme-linked immunosorbent assay, while placental levels of active caspase-3 were determined by western blotting. Human umbilical vein endothelial cells (HUVECs) were cultured with STBMs and the proliferation and apoptosis rates of the HUVECs assessed.
Results: Levels of STBMs in the early-onset group (71.2 ± 20.7 ng/mL) were significantly higher than those detected in the late-onset group (41.9 ± 29.7 ng/mL) and the control group (26.3 ± 11.2 ng/mL) (P<0.05). The amount of active caspase-3 was increased in the early-onset (0.85 ± 0.61) and late-onset groups (0.77 ± 0.46) relative to the control group (0.32 ± 0.15) (P<0.05). Proliferation of HUVECs was inhibited, while apoptosis was elevated, following co-culture with STBMs.
Conclusion: Shedding of STBMs into the maternal circulation occurs in greater amounts in early-onset pre-eclampsia than in late-onset pre-eclampsia.
Copyright © 2012 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All rights reserved.
Similar articles
-
[Study on apoptosis of placental syncytiotrophoblast in patients with severe preeclampsia].Zhonghua Fu Chan Ke Za Zhi. 2010 Oct;45(10):750-3. Zhonghua Fu Chan Ke Za Zhi. 2010. PMID: 21176555 Chinese.
-
Excess syncytiotrophoblast microparticle shedding is a feature of early-onset pre-eclampsia, but not normotensive intrauterine growth restriction.Placenta. 2006 Jan;27(1):56-61. doi: 10.1016/j.placenta.2004.11.007. Epub 2005 Jan 25. Placenta. 2006. PMID: 16310038
-
[Expression of various subtypes of human leukocyte antigen-G in placenta of patients complicated with severe pre-eclampsia].Zhonghua Fu Chan Ke Za Zhi. 2012 Jan;47(1):29-32. Zhonghua Fu Chan Ke Za Zhi. 2012. PMID: 22455690 Chinese.
-
The functions of microparticles in pre-eclampsia.Semin Thromb Hemost. 2011 Mar;37(2):146-52. doi: 10.1055/s-0030-1270342. Epub 2011 Mar 2. Semin Thromb Hemost. 2011. PMID: 21370216 Review.
-
Placental microparticles, DNA, and RNA in preeclampsia.Hypertens Pregnancy. 2011;30(3):364-75. doi: 10.3109/10641951003599571. Epub 2010 Dec 21. Hypertens Pregnancy. 2011. PMID: 21174588 Review.
Cited by
-
Epigenetic drug screening for trophoblast syncytialization reveals a novel role for MLL1 in regulating fetoplacental growth.BMC Med. 2024 Feb 5;22(1):57. doi: 10.1186/s12916-024-03264-8. BMC Med. 2024. PMID: 38317232 Free PMC article.
-
Potential Roles of microRNAs for Assessing Cardiovascular Risk in Pre-Eclampsia-Exposed Postpartum Women and Offspring.Int J Mol Sci. 2023 Nov 28;24(23):16842. doi: 10.3390/ijms242316842. Int J Mol Sci. 2023. PMID: 38069164 Free PMC article. Review.
-
Preliminary evidence that blocking the uptake of placenta-derived preeclamptic extracellular vesicles protects the vascular endothelium and prevents vasoconstriction.Sci Rep. 2023 Oct 27;13(1):18425. doi: 10.1038/s41598-023-45830-9. Sci Rep. 2023. PMID: 37891193 Free PMC article.
-
Pravastatin reduces plasma levels of extracellular vesicles in pregnancies at high risk of term preeclampsia.Front Pharmacol. 2023 Jun 22;14:1166123. doi: 10.3389/fphar.2023.1166123. eCollection 2023. Front Pharmacol. 2023. PMID: 37426825 Free PMC article.
-
Pre-eclampsia.Nat Rev Dis Primers. 2023 Feb 16;9(1):8. doi: 10.1038/s41572-023-00417-6. Nat Rev Dis Primers. 2023. PMID: 36797292 Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials