Oral DNA vaccination of rainbow trout, Oncorhynchus mykiss (Walbaum), against infectious haematopoietic necrosis virus using PLGA [Poly(D,L-Lactic-Co-Glycolic Acid)] nanoparticles
- PMID: 22324344
- DOI: 10.1111/j.1365-2761.2011.01338.x
Oral DNA vaccination of rainbow trout, Oncorhynchus mykiss (Walbaum), against infectious haematopoietic necrosis virus using PLGA [Poly(D,L-Lactic-Co-Glycolic Acid)] nanoparticles
Abstract
A DNA vaccine against infectious haematopoietic necrosis virus (IHNV) is effective at protecting rainbow trout, Oncorhynchus mykiss, against disease, but intramuscular injection is required and makes the vaccine impractical for use in the freshwater rainbow trout farming industry. Poly (D,L-lactic-co-glycolic acid) (PLGA) is a U.S. Food and Drug Administration (FDA) approved polymer that can be used to deliver DNA vaccines. We evaluated the in vivo absorption of PLGA nanoparticles containing coumarin-6 when added to a fish food pellet. We demonstrated that rainbow trout will eat PLGA nanoparticle coated feed and that these nanoparticles can be detected in the epithelial cells of the lower intestine within 96 h after feeding. We also detected low levels of gene expression and anti-IHNV neutralizing antibodies when fish were fed or intubated with PLGA nanoparticles containing IHNV G gene plasmid. A virus challenge evaluation suggested a slight increase in survival at 6 weeks post-vaccination in fish that received a high dose of the oral vaccine, but there was no difference when additional fish were challenged at 10 weeks post-vaccination. The results of this study suggest that it is possible to induce an immune response using an orally delivered DNA vaccine, but the current system needs improvement.
© 2012 Blackwell Publishing Ltd.
Similar articles
-
An oral DNA vaccine against infectious haematopoietic necrosis virus (IHNV) encapsulated in alginate microspheres induces dose-dependent immune responses and significant protection in rainbow trout (Oncorrhynchus mykiss).Fish Shellfish Immunol. 2015 Aug;45(2):877-88. doi: 10.1016/j.fsi.2015.05.045. Epub 2015 Jun 6. Fish Shellfish Immunol. 2015. PMID: 26054788
-
Evaluation of dual nasal delivery of infectious hematopoietic necrosis virus and enteric red mouth vaccines in rainbow trout (Oncorhynchus mykiss).Vaccine. 2015 Feb 4;33(6):771-6. doi: 10.1016/j.vaccine.2014.12.055. Epub 2015 Jan 3. Vaccine. 2015. PMID: 25562788
-
Specificity of DNA vaccines against the U and M genogroups of infectious hematopoietic necrosis virus (IHNV) in rainbow trout (Oncorhynchus mykiss).Fish Shellfish Immunol. 2011 Jul;31(1):43-51. doi: 10.1016/j.fsi.2011.03.003. Epub 2011 Mar 6. Fish Shellfish Immunol. 2011. PMID: 21385613
-
DNA vaccines as a tool for analysing the protective immune response against rhabdoviruses in rainbow trout.Fish Shellfish Immunol. 2002 May;12(5):439-53. doi: 10.1006/fsim.2002.0422. Fish Shellfish Immunol. 2002. PMID: 12194454 Review.
-
Strategies and hurdles using DNA vaccines to fish.Vet Res. 2014 Feb 19;45(1):21. doi: 10.1186/1297-9716-45-21. Vet Res. 2014. PMID: 24552235 Free PMC article. Review.
Cited by
-
Nanoencapsulated Aquafeeds and Current Uses in Fisheries/Shrimps: A Review.Appl Biochem Biotechnol. 2023 Nov;195(11):7110-7131. doi: 10.1007/s12010-023-04418-9. Epub 2023 Mar 8. Appl Biochem Biotechnol. 2023. PMID: 36884191 Review.
-
Tilapia Lake Virus Vaccine Development: A Review on the Recent Advances.Vaccines (Basel). 2023 Jan 23;11(2):251. doi: 10.3390/vaccines11020251. Vaccines (Basel). 2023. PMID: 36851129 Free PMC article. Review.
-
Polymeric Nanoparticles for Inhaled Vaccines.Polymers (Basel). 2022 Oct 21;14(20):4450. doi: 10.3390/polym14204450. Polymers (Basel). 2022. PMID: 36298030 Free PMC article. Review.
-
Antibiotic resistance in aquaculture and aquatic organisms: a review of current nanotechnology applications for sustainable management.Environ Sci Pollut Res Int. 2022 Oct;29(46):69241-69274. doi: 10.1007/s11356-022-22319-y. Epub 2022 Aug 15. Environ Sci Pollut Res Int. 2022. PMID: 35969340 Free PMC article. Review.
-
Immunological Effects of Recombinant Lactobacillus casei Expressing IHNV G Protein and Rainbow Trout (Oncorhynchus mykiss) Chemokine CK6 as an Oral Vaccine.Front Immunol. 2022 Jun 16;13:927443. doi: 10.3389/fimmu.2022.927443. eCollection 2022. Front Immunol. 2022. PMID: 35784302 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources