A standardized protocol for repeated social defeat stress in mice
- PMID: 21799487
- PMCID: PMC3220278
- DOI: 10.1038/nprot.2011.361
A standardized protocol for repeated social defeat stress in mice
Erratum in
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Corrigendum: a standardized protocol for repeated social defeat stress in mice.Nat Protoc. 2015 Apr;10(4):643. doi: 10.1038/nprot0415-644a. Epub 2015 Mar 26. Nat Protoc. 2015. PMID: 25811900 No abstract available.
Abstract
A major impediment to novel drug development has been the paucity of animal models that accurately reflect symptoms of affective disorders. In animal models, prolonged social stress has proven to be useful in understanding the molecular mechanisms underlying affective-like disorders. When considering experimental approaches for studying depression, social defeat stress, in particular, has been shown to have excellent etiological, predictive, discriminative and face validity. Described here is a protocol whereby C57BL/6J mice that are repeatedly subjected to bouts of social defeat by a larger and aggressive CD-1 mouse results in the development of a clear depressive-like syndrome, characterized by enduring deficits in social interactions. Specifically, the protocol consists of three important stages, beginning with the selection of aggressive CD-1 mice, followed by agonistic social confrontations between the CD-1 and C57BL/6J mice, and concluding with the confirmation of social avoidance in subordinate C57BL/6J mice. The automated detection of social avoidance allows a marked increase in throughput, reproducibility and quantitative analysis. This protocol is highly adaptable, but in its most common form it requires 3-4 weeks for completion.
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