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. 2011 Apr;98(2):311-5.
doi: 10.1016/j.pbb.2011.01.014. Epub 2011 Jan 28.

Fluoxetine prevents 8-OH-DPAT-induced hyperphagia in Fischer inbred rats

Chandra Suma Johnson Miryala  1 , Navin Maswood, Lynda Uphouse
Affiliations

Fluoxetine prevents 8-OH-DPAT-induced hyperphagia in Fischer inbred rats

Chandra Suma Johnson Miryala et al. Pharmacol Biochem Behav. 2011 Apr.

Abstract

Ovariectomized, Fischer rats were hormonally primed with 10 μg estradiol benzoate and 50 μg progesterone or were treated with the sesame seed oil vehicle. Food intake was measured 2 h and 24 h after treatment with 0.25 mg/kg of the 5-HT(1A) receptor agonist, (±)-8-hydroxy 2-(di-n-propylamino) tetralin (8-OH-DPAT), 5 mg/kg of the selective serotonin reuptake inhibitor, fluoxetine, or their combination. Consistent with prior studies, two hour food intake of rats given fluoxetine and 8-OH-DPAT did not differ from vehicle controls. 8-OH-DPAT-induced hyperphagia, evident at 2 h, was blocked by co-treatment with fluoxetine. However, in contrast to prior studies, 5 mg/kg fluoxetine, alone, had only modest effects on food intake. Differences in our experimental protocols and/or the strain of rat may account for the lower anorectic response to fluoxetine. Nevertheless, the absence of a significant response to fluoxetine, alone, coupled with the drug's attenuation of the hyperphagic effect of 8-OH-DPAT, leads to the suggestion that the behavioral response to the combined treatment is more complex than that of simple additivity. Consistent with this suggestion, 24 h food intake of rats given 8-OH-DPAT and fluoxetine was lower than that of vehicle or 8-OH-DPAT-treated rats.

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Figures

Figure 1
Figure 1
Two hr food intake in EP and OO rats Ovariectomized rats were hormonally primed with 10 ig estradiol benzoate followed by 50 ig progesterone (EP rats) or received control injections with sesame seed oil (OO rats). Data are the mean ± S.E. 2 hr food intake for vehicle-treated rats (VEH), rats injected with 0.25 mg 8-OH-DPAT (DPAT), rats injected with 5 mg/kg fluoxetine (FLX), and rats injected with 5 mg/kg fluoxetine and 0.25 mg/kg 8-OH-DPAT (DPAT/FLX). For EP and OO rats, N's for vehicle, 8-OH-DPAT, fluoxetine, and 8-OH-DPAT/fluoxetine, respectively, were 10, 9,10, 10 and 9, 9, 11, 11. *indicates significant difference from rats treated only with 8-OH-DPAT. ** indicates significant difference from vehicle-treated rats.
Figure 2
Figure 2
Twenty-four hr food intake in EP and OO rats Ovariectomized rats were hormonally primed with 10 ig estradiol benzoate followed by 50 ig progesterone (EP rats) or received control injections with sesame seed oil (OO rats). Data are the mean ± S.E. 24 hr food intake for vehicle-treated rats (VEH), rats injected with 0.25 mg 8-OH-DPAT (DPAT), rats injected with 5 mg/kg fluoxetine (FLX), and rats injected with 5 mg/kg fluoxetine and 0.25 mg/kg 8-OH-DPAT (DPAT/FLX). For EP and OO rats, N's for vehicle, 8-OH-DPAT, fluoxetine, and 8-OH-DPAT/fluoxetine, respectively, were 10, 9, 10, 10 and 9, 9, 11, 11. *indicates significant difference from rats treated only with 8-OH-DPAT. ** indicates significant difference from vehicle-treated rats.
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