Noninvasive assessment of dermal carotenoids as a biomarker of fruit and vegetable intake
- PMID: 20685953
- PMCID: PMC3133234
- DOI: 10.3945/ajcn.2010.29707
Noninvasive assessment of dermal carotenoids as a biomarker of fruit and vegetable intake
Abstract
Background: Resonance Raman spectroscopy (RRS) has been suggested as a feasible method for noninvasive carotenoid measurement of human skin. However, before RRS measures of dermal carotenoids can be used as a biomarker, data on intra- and intersubject variability and validity are needed.
Objective: The purpose of this study was to evaluate the reproducibility and validity of RRS measures of dermal total carotenoids and lycopene in humans.
Design: In study 1, 74 men and women with diverse skin pigmentation were recruited. RRS measures of the palm, inner arm, and outer arm were obtained at baseline, 1 wk, 2 wk, 1 mo, 3 mo, and 6 mo (to maximize seasonal variation). The RRS device used visible light at 488 nm to estimate total carotenoids and at 514 nm to estimate lycopene. Reproducibility was assessed by intraclass correlation coefficients (ICCs). In study 2, we recruited 28 subjects and assessed dietary carotenoid intake, obtained blood for HPLC analyses, performed RRS measures of dermal carotenoid status, and performed dermal biopsies (3-mm punch biopsy) with dermal carotenoids assessed by HPLC.
Results: ICCs for total carotenoids across time were 0.97 (palm), 0.95 (inner arm), and 0.93 (outer arm). Total dermal carotenoids assessed by RRS were significantly correlated with total dermal carotenoids assessed by HPLC of dermal biopsies (r = 0.66, P = 0.0001). Similarly, lycopene assessed by RRS was significantly correlated with lycopene assessed by HPLC of dermal biopsies (r = 0.74, P < 0.0001).
Conclusion: RRS is a feasible and valid method for noninvasively assessing dermal carotenoids as a biomarker for studies of nutrition and health.
Figures
Similar articles
-
Concurrent validity of skin carotenoid status as a concentration biomarker of vegetable and fruit intake compared to multiple 24-h recalls and plasma carotenoid concentrations across one year: a cohort study.Nutr J. 2019 Nov 21;18(1):78. doi: 10.1186/s12937-019-0500-0. Nutr J. 2019. PMID: 31752882 Free PMC article.
-
Single v. multiple measures of skin carotenoids by resonance Raman spectroscopy as a biomarker of usual carotenoid status.Br J Nutr. 2013 Sep 14;110(5):911-7. doi: 10.1017/S000711451200582X. Epub 2013 Jan 28. Br J Nutr. 2013. PMID: 23351238 Free PMC article.
-
Optical assessment of skin carotenoid status as a biomarker of vegetable and fruit intake.Arch Biochem Biophys. 2018 May 15;646:46-54. doi: 10.1016/j.abb.2018.03.033. Epub 2018 Mar 27. Arch Biochem Biophys. 2018. PMID: 29601824 Free PMC article.
-
Resonance Raman spectroscopic evaluation of skin carotenoids as a biomarker of carotenoid status for human studies.Arch Biochem Biophys. 2013 Nov 15;539(2):163-70. doi: 10.1016/j.abb.2013.06.007. Epub 2013 Jun 30. Arch Biochem Biophys. 2013. PMID: 23823930 Free PMC article. Review.
-
Resonance Raman detection of carotenoid antioxidants in living human tissue.J Biomed Opt. 2005 Nov-Dec;10(6):064028. doi: 10.1117/1.2139974. J Biomed Opt. 2005. PMID: 16409093 Free PMC article. Review.
Cited by
-
Evaluation of a Commercial Device Based on Reflection Spectroscopy as an Alternative to Resonance Raman Spectroscopy in Measuring Skin Carotenoid Levels: Randomized Controlled Trial.Sensors (Basel). 2023 Sep 4;23(17):7654. doi: 10.3390/s23177654. Sensors (Basel). 2023. PMID: 37688110 Free PMC article. Clinical Trial.
-
A Narrative Review of Factors Associated with Skin Carotenoid Levels.Nutrients. 2023 Apr 30;15(9):2156. doi: 10.3390/nu15092156. Nutrients. 2023. PMID: 37432294 Free PMC article. Review.
-
Cost analysis and cost effectiveness of a subsidized community supported agriculture intervention for low-income families.Int J Behav Nutr Phys Act. 2023 Jul 10;20(1):84. doi: 10.1186/s12966-023-01481-7. Int J Behav Nutr Phys Act. 2023. PMID: 37430305 Free PMC article.
-
Systematic Review of Reflection Spectroscopy-Based Skin Carotenoid Assessment in Children.Nutrients. 2023 Mar 7;15(6):1315. doi: 10.3390/nu15061315. Nutrients. 2023. PMID: 36986046 Free PMC article.
-
Raman Spectroscopy of Carotenoid Compounds for Clinical Applications-A Review.Molecules. 2022 Dec 17;27(24):9017. doi: 10.3390/molecules27249017. Molecules. 2022. PMID: 36558154 Free PMC article. Review.
References
-
- Institute of Medicine, National Academy of Sciences, Food and Nutrition Board, Panel on Dietary Antioxidants and Related Compounds Dietary reference intakes for vitamin C, vitamin E, selenium, and carotenoids. Washington, DC: National Academies Press, 2000 - PubMed
-
- Peng YM, Peng YS, Lin Y, Moon T, Roe DJ, Ritenbaugh C. Concentrations and plasma-tissue-diet relationships of carotenoids, retinoids, and tocopherols in humans. Nutr Cancer 1995;23:233–46 - PubMed
-
- McEligot AJ, Rock CL, Flatt SW, Newman V, Faerber S, Pierce JP. Plasma carotenoids are biomarkers of long-term high vegetable intake in women with breast cancer. J Nutr 1999;129:2258–63 - PubMed
-
- Lanza E, Schatzkin A, Daston C, et al. Implementation of a 4-y, high-fiber, high-fruit-and-vegetable, low-fat dietary intervention: results of dietary changes in the Polyp Prevention Trial. Am J Clin Nutr 2001;74:387–401 - PubMed
-
- World Cancer Research Fund, American Institution for Cancer Research Food, nutrition, physical activity, and the prevention of cancer: a global perspective. The Second Expert Report. Washington, DC: AICR, 2007
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources