A large scale laboratory cage trial of Aedes densonucleosis virus (AeDNV)
- PMID: 20496587
- DOI: 10.1603/me09157
A large scale laboratory cage trial of Aedes densonucleosis virus (AeDNV)
Abstract
Aedes aegypti (L.) (Diptera: Culicidae) the primary vector of dengue viruses (DENV1-4), oviposit in and around human dwellings, including sites difficult to locate, making control of this mosquito challenging. We explored the efficacy and sustainability of Aedes Densonucleosis Virus (AeDNV) as a biocontrol agent for Ae. aegypti in and among oviposition sites in large laboratory cages (> 92 m3) as a prelude to field trials. Select cages were seeded with AeDNV in a single oviposition site (OPS) with unseeded OPSs established at varied distances. Quantitative real-time polymerase chain reaction was used to track dispersal and accumulation of AeDNV among OPSs. All eggs were collected weekly from each cage and counted. We asked: (1) Is AeDNV dispersed over varying distances and can it accumulate and persist in novel OPSs? (2) Are egg densities reduced in AeDNV treated populations? AeDNV was dispersed to and sustained in novel OPSs. Virus accumulation in OPSs was positively correlated with egg densities and proximity to the initial infection source affected the timing of dispersal and maintenance of viral titers. AeDNV did not significantly reduce Ae. aegypti egg densities. The current study documents that adult female Ae. aegypti oviposition behavior leads to successful viral dispersal from treated to novel containers in large-scale cages; however, the AeDNV titers reached were not sufficient to reduce egg densities.
Similar articles
-
Aedes aegypti densonucleosis virus amplifies, spreads, and reduces host populations in laboratory cage studies.J Med Entomol. 2009 Jul;46(4):909-18. doi: 10.1603/033.046.0425. J Med Entomol. 2009. PMID: 19645297
-
Infection and pathogenicity of the mosquito densoviruses AeDNV, HeDNV, and APeDNV in Aedes aegypti mosquitoes (Diptera: Culicidae).J Econ Entomol. 2004 Dec;97(6):1828-35. doi: 10.1093/jee/97.6.1828. J Econ Entomol. 2004. PMID: 15666733
-
Evaluation of mosquito densoviruses for controlling Aedes aegypti (Diptera: Culicidae): variation in efficiency due to virus strain and geographic origin of mosquitoes.Am J Trop Med Hyg. 2008 May;78(5):784-90. Am J Trop Med Hyg. 2008. PMID: 18458314
-
Mosquito densonucleosis viruses cause dramatically different infection phenotypes in the C6/36 Aedes albopictus cell line.Virology. 2005 Jul 5;337(2):253-61. doi: 10.1016/j.virol.2005.04.037. Virology. 2005. PMID: 15919104
-
[Aedes albopictus, vector of chikungunya and dengue viruses in Reunion Island: biology and control].Parasite. 2008 Mar;15(1):3-13. doi: 10.1051/parasite/2008151003. Parasite. 2008. PMID: 18416242 Review. French.
Cited by
-
Direct mosquito feedings on dengue-2 virus-infected people reveal dynamics of human infectiousness.PLoS Negl Trop Dis. 2023 Sep 1;17(9):e0011593. doi: 10.1371/journal.pntd.0011593. eCollection 2023 Sep. PLoS Negl Trop Dis. 2023. PMID: 37656759 Free PMC article.
-
New self-sexing Aedes aegypti strain eliminates barriers to scalable and sustainable vector control for governments and communities in dengue-prone environments.Front Bioeng Biotechnol. 2022 Oct 25;10:975786. doi: 10.3389/fbioe.2022.975786. eCollection 2022. Front Bioeng Biotechnol. 2022. PMID: 36394032 Free PMC article.
-
Driving down malaria transmission with engineered gene drives.Front Genet. 2022 Oct 19;13:891218. doi: 10.3389/fgene.2022.891218. eCollection 2022. Front Genet. 2022. PMID: 36338968 Free PMC article. Review.
-
Elimination of a closed population of the yellow fever mosquito, Aedes aegypti, through releases of self-limiting male mosquitoes.PLoS Negl Trop Dis. 2022 May 16;16(5):e0010315. doi: 10.1371/journal.pntd.0010315. eCollection 2022 May. PLoS Negl Trop Dis. 2022. PMID: 35576193 Free PMC article.
-
Genetic pest management and the background genetics of release strains.Philos Trans R Soc Lond B Biol Sci. 2021 Feb 15;376(1818):20190805. doi: 10.1098/rstb.2019.0805. Epub 2020 Dec 28. Philos Trans R Soc Lond B Biol Sci. 2021. PMID: 33357053 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources