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Case Reports
. 2008 Sep-Oct;25(9-10):437-43.
doi: 10.1007/s10815-008-9254-x. Epub 2008 Oct 15.

Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: a case report

Richard J Kordus  1 , Robert L Price, Jeffrey M Davis, Gail F Whitman-Elia
Affiliations
Case Reports

Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: a case report

Richard J Kordus et al. J Assist Reprod Genet. 2008 Sep-Oct.

Abstract

Purpose: To describe the ultrastructure of spermatozoa from a patient with complete asthenozoospermia that resulted in live births following blastocyst culture.

Materials and methods: Analyses of spermatozoa from a 36 year old patient were performed using light and electron microscopy. The hypo-osmotic swelling test was used to select spermatozoa for intracytoplasmic sperm injection. Embryos were cultured to the blastocyst stage.

Results: 100% of the spermatozoa had dynein arm deficiency with secondary defects varying from 3-17%. Six oocytes were injected; five fertilized normally and one was digynic. All five zygotes formed good quality blastocysts. Three blastocysts were cryopreserved and two blastocysts were transferred. Twin females were born at 37 weeks.

Conclusions: The hypo-osmotic swelling test can be used to select viable immotile ejaculated spermatozoa from a patient with dynein arm deficiency and can produce excellent fertilization rates and blastocyst development resulting in live births.

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Figures

Fig. 1
Fig. 1
Transmission electron micrograph of a cross-section of a spermatozoon flagellum with almost complete abscence of dynein arms at 200,000× magnification. Note the presence of a truncated outer dynein arm (blue arrow) and inner dynein arms (red arrow)
Fig. 2
Fig. 2
Transmission electron micrograph of various spermatozoa from the patient with PCD. A) A cross section through a mid-piece region of flagellum. B) Three axonemes surrounded by the same outer membrane. Notice the partial axonemal disruption (Arrow). C) Transverse section of a spermatozoon. D) Same spermatozoon flagellum at a higher magnification. Notice the disruption of the mitochondrial sheath (Arrow)
See this image and copyright information in PMC

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