Pancreatic acinar and islet cell infection by low-dose SV40 administration
- PMID: 18437088
- DOI: 10.1097/MPA.0b013e31815d349b
Pancreatic acinar and islet cell infection by low-dose SV40 administration
Abstract
Objectives: Viral vector uptake into the pancreas is rare. The few viral vectors reported to transduce in vivo pancreatic islets after systemic injection required additional physical measures, such as direct pancreatic injection or hepatic vessel clamping. Because pancreatic islet uptake of the human polyomavirus family member BK virus was previously reported in hamsters after systemic administration, we hypothesized that SV40, a polyomavirus member remarkably similar to BK virus, may also infect the pancreas.
Methods: We injected intravenously a low dose of SV40, unaided by any other physical or chemical means, and evaluated viral uptake by pancreatic islets and pancreatic exocrine tissue via polymerase chain reaction, Western blot, electron microscopy, immunofluorescent microscopy, and protein A-gold immunocytochemistry.
Results: Pancreatic uptake of SV40 was comparable to other major organs (ie, liver and spleen). SV40 viral particles were detected in both pancreatic islets and acini. In pancreatic islets, all islet cell types were infected by SV40, albeit the infection rate of glucagon-producing alpha cells surpassed beta- and delta-islet cells. Low-dose SV40 administration was not sufficient to induce heterologous gene expression in the pancreas.
Conclusions: Our study shows that pancreatic islet and acinar cell uptake of SV40 is feasible with a single, low-dose intravenous injection. However, this dose did not result in gene delivery into the murine pancreas.
Similar articles
-
Immunohistochemical demonstration of bovine viral diarrhoea virus antigen in the pancreatic islet cells of cattle with insulin-dependent diabetes mellitus.J Comp Pathol. 1999 Aug;121(2):149-57. doi: 10.1053/jcpa.1999.0312. J Comp Pathol. 1999. PMID: 10405307
-
Morphologic and immunocytochemical study of young dogs with diabetes mellitus associated with pancreatic islet hypoplasia.Am J Vet Res. 1988 Sep;49(9):1577-81. Am J Vet Res. 1988. PMID: 3066245
-
Topographical associations between islet endocrine cells and duct epithelial cells in the adult human pancreas.Clin Endocrinol (Oxf). 2008 Sep;69(3):400-6. doi: 10.1111/j.1365-2265.2008.03190.x. Epub 2008 Jan 21. Clin Endocrinol (Oxf). 2008. PMID: 18221396
-
Is there a role for SV40 in human cancer?J Clin Oncol. 2006 Sep 10;24(26):4356-65. doi: 10.1200/JCO.2005.03.7101. J Clin Oncol. 2006. PMID: 16963733 Review.
-
[Systemic diseases and the pancreas].Verh Dtsch Ges Pathol. 1987;71:77-83. Verh Dtsch Ges Pathol. 1987. PMID: 3326347 Review. German. No abstract available.
Cited by
-
Safety and Efficacy of AAV Retrograde Pancreatic Ductal Gene Delivery in Normal and Pancreatic Cancer Mice.Mol Ther Methods Clin Dev. 2017 Sep 30;8:8-20. doi: 10.1016/j.omtm.2017.09.006. eCollection 2018 Mar 16. Mol Ther Methods Clin Dev. 2017. PMID: 29349096 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Medical